| 颜巧丽，邓福明，陈卫军，赵松林.直投式木葡萄糖醋酸杆菌发酵剂的保护剂筛选研究[J].广东农业科学,2013,40(8):105-109 |
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直投式木葡萄糖醋酸杆菌发酵剂的保护剂筛选研究 |
| Research on screening of freeze-dried protectants for DVS Gluconacetobacter xylinum |
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| DOI： |
| 中文关键词: 木葡萄糖醋酸杆菌 保护剂 冷冻干燥 细菌纤维素 |
| 英文关键词: Gluconacetobacter xylinum protectant freeze-drying Nata |
| 基金项目:国家重大科技成果转化项目；海南省重大科技项目（ZDZX2013011） |
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| 中文摘要: |
| 利用冷冻干燥技术制备一种直投式木葡萄糖醋酸杆菌的复合保护剂，先采用单因素试验优选出10%脱脂奶粉、5%海藻糖、1%谷氨酸可作为木葡糖醋酸杆菌发酵剂的单一保护剂，然后采用响应面分析法优化了直投式木葡萄糖醋酸杆菌复合保护剂中脱脂奶粉、海藻糖、谷氨酸的浓度和配比。结果表明，当复合冻干保护剂中含脱脂乳6.4%、海藻糖0.075%、谷氨酸0.575%时，木葡萄糖醋酸杆菌冻干后细胞的存活率最高、达93.346%。对比试验表明，添加复合冻干保护剂的发酵剂与同浓度的活菌液体发酵剂的细菌纤维素产率差异不显著。 |
| 英文摘要: |
| A DVS Gluconacetobacter xylinum composite protectve agent was developed by using freeze drying echnique. Firstly, 10% skimmed milk, 5% trehalose, 1% glutamate were screened respectively as the protective agent of G.xylinum through single factor test. Furtherly, the formula of cyprotectants for G. xylinum was optimized by central composite design (CCD). The result showed that the survival rate of the freeze-dried G. xylinum was up to a maximum of 93.346% when the cryoprotectant contained 6.4% skimmed milk, 0.075% trehalose and 0.575% glutamate. Finally, the comparative trial showed that the yields of Nata were not significant among in the starter culture adding the compound freeze-dried cryoprotectant and in the starter culture with the same concentration of living bacterium liquid. |
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