| 闫秋良，翟洪军，马惠海，金海国，李双双，曹阳，武斌，赵玉民.绵羊LDHAL6A基因的电子克隆与生物信息学预测[J].广东农业科学,2013,40(10):155-158 |
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绵羊LDHAL6A基因的电子克隆与生物信息学预测 |
| In silico cloning and bioinformatic prediction of sheep LDHAL6A gene |
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| DOI： |
| 中文关键词: 绵羊 LDHAL6A基因 电子克隆 生物信息学 |
| 英文关键词: sheep LDHAL6A gene in silico cloning bioinformatics |
| 基金项目:吉林省人力资源和社会保障厅项目 |
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| 中文摘要: |
| 以人LDHAL6A基因的cDNA序列为探针，利用生物信息学方法，对绵羊LDHAL6A基因进行了电子克隆，并对推导出LDHAL6A基因编码的蛋白结构与性质进行了预测。结果表明：绵羊LDHAL6A基因的cDNA序列全长为1798bp，包括999 bp的编码区和149bp的5ˊ非翻译区（5ˊUTR）、650 bp的3ˊ非翻译区序列（3ˊUTR），编码合成332个氨基酸的多肽；其编码蛋白属疏水性蛋白，不存在信号肽及跨膜结构，定位于内质网上；无规则卷曲、琢-螺旋是绵羊LDHAL6A蛋白主要的二级结构元件。绵羊LDHAL6A基因编码蛋白可能在精子生成过程中起重要作用。 |
| 英文摘要: |
| Sheep EST sequences were aligned using human LDHAL6A cDNA sequence as a probe from sheep EST database in NCBI website, and sheep LDHAL6A gene was obtained by in silico cloning. The structure and property of the amino acid encoded by LDHAL6A gene were predicted. Results showed that sheep LDHAL6A cDNA sequence was 1 798 bp long, and it contained 149 bp of 5ˊUTR, 650 bp of 3ˊUTR as well as 999 bp of open reading frame (ORF) that encoded a 332 amino acid peptide. The protein encoded by sheep LDHAL6A gene had no signal peptide, and no transmembrane domains, it was a hydrophilic protein which was located in endoplasmic reticulum. The secondary structures were mainly composed of Alpha helix and random coil. Sequence analysis indicated that it mighht play a crucial role in the process of spermatogenesis. |
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