| 韩伯涛，盖颖，蒋湘.正义、反义、干涉调控肉桂酰辅酶A脱氢酶基因对烟草的影响[J].广东农业科学,2013,40(11):140-144 |
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正义、反义、干涉调控肉桂酰辅酶A脱氢酶基因对烟草的影响 |
| Regulation of Cinnamoyl-CoA reductase by sense，antisense and RNAi method to tobacco |
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| DOI： |
| 中文关键词: 肉桂酰辅酶A脱氢酶 木质素 转基因 |
| 英文关键词: Cinnamoyl-CoA reductase lignin Transgene |
| 基金项目:国家863计划项目（2011AA100203） |
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| 摘要点击次数: 1276 |
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| 中文摘要: |
| 肉桂酰辅酶A还原酶（Cinnamoyl-CoA reductase（CCR）是在木质素合成途径中起到关键作用的酶，负责催化肉桂酰辅酶A类物质的还原反应生成相应的醛类化合物。分离得到毛白杨CCR基因的cDNA。将CCR基因以正义、反义和干涉的形式，通过农杆菌介导的方法转入烟草中，Southern杂交以确定转基因成功。在获得的转基因植株中，所有下调基因的植株都表现出可溶性酚酸含量下降，木质素含量也有明显的下降趋势，纤维素含量则有上升，可能是对木质素含量下降的一种代偿性增长。植物细胞壁组分的含量变化表明CCR基因可能是一个控制木质素代谢途径的一个很好的调控点。 |
| 英文摘要: |
| Lignin is the second abundant organics in the terrestrial vascular plants, which is crucial for the plants to adapt the environment during evolution. Cinnamoyl -CoA reductase (CCR) is a key enzyme in the lignin biosynthesis pathway, catalyze the conversion of cinnamoyl-CoA esters to their cinnamaldehydes respectively. A 1017bp cDNA of CCR was isolated from populus tomentosa.Sense, antisense and a full-length repeat constructs of CCR were introduced into tobacco (Nicotiana tabacum) to regulate the gene expression. The transgenic plants exhibited correlation between the gene transcript and the enzyme activity. All the down-regulation plants showed a lower transcript levels and catalyze ability. The accumulation of soluble phenolic acid in cell wall of the down-regulated lines indicates that the carbon flow of lignin synthesis might be blocked. Lignin content was evaluated by the standard Klason lignin method and Acetyl bromide method, The severely down-regulated line A1 showed almost 50% decrease either in Klason lignin or bromoacetylated lignin. The changes of the cell wall properties in transformants suggested that the CCR might be a good control point in lignin pathway regulation. |
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