| 李育川，李维莉，莫丽玲，靳松.小桐子枝叶抑菌活性部位分离及其GC-MS分析[J].广东农业科学,2013,40(18):91-93 |
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小桐子枝叶抑菌活性部位分离及其GC-MS分析 |
| Isolation and GC-MS analysis of antibacterial activity franctions from Japropha curcas L. branch-leaves |
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| DOI： |
| 中文关键词: 小桐子枝叶 抑菌活性物质 分离鉴定 GC-MS |
| 英文关键词: J. curcas fruit shell antibacterial activity components isolation and identification GC-MS |
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| 摘要点击次数: 1143 |
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| 中文摘要: |
| 以具有抑菌活性的小桐子枝叶乙醇粗提物的石油醚萃取相为试验材料，采用硅胶柱反复柱层析，结合PTLC分离制备，同时对各阶段分离产物进行活性追踪，筛选出具有抗菌作用的活性部位AJ3，采用GC-MS分析，初步鉴定出活性部位的主体成分。结果表明，AJ3组分在浓度为0.8 mg/mL时，对白菜黑斑病菌[Alternaria brassicae (Berk.) Sacc.]和小麦赤霉病菌（Fusariumgraminearum）菌丝72 h生长抑制率均大于85%，与广谱性杀菌剂多菌灵（carbendazim）无显著差异，表现出极高的抑菌活性。对AJ3组分进行GC-MS分析，鉴定出含量最高的10个化合物，它们占总量的83.52%。含量最高的前5位成分依次是3-已(烷)基过氧氢酸、2-环,3 [1,3-苯二氧基-5-乙基]-2-丙烯酸]2,3-环氧-2-甲基已烷尧2,4-二甲基-3-已醇和苯乙烯，5种主体成分的相对总含量为64.67%，主体成分属酚酸类，单萜类化合物。 |
| 英文摘要: |
| To creened antibacterial activity components from Jatropha Curcas L. branch- leaves. Using alcohol crude extract of petroleum ether partitioned extracts in J. curcas L. branch- leaves as an experimental material by using silica gel column chromatography,combining with PTLC preparation. At same time, each stage of separated products was carried out by tracking activity, and analyzed by GCMS,combined with sceening the main compositions of exhibited active components. The results show that with the concentration of AJ3 colony of 0.8 mg/mL, the growth inhibition rates of Alternaria brassicae （Berk.）Sacc. and Fusarium graminearum, both exceeded 85%,showing no significant differences with carbendazim, it showed that the antibacterial activity was very high. The constituents analysis of AJ3 by using GC-MS showed that, 10 maximum contents were identification, with total amount up to 83.52%, the first five maximum contents were 3-Hexylhydroperoxide, 2-Cyc,3-[1,3-benzodioxol-5-ly]-2-Propenoic acid, Hexane, 2-3-Epoxy-2-methyl-ethane, 2,4-Dimethyl-3-hexanol and Styrene, the total relative contents were 64.67%, as the main components were phenolic acides and monoterpenes. |
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