| 彭妹1 王立升1 刘帅兵2 李婉媚1 薛井中2.白背叶根药材的HPLC指纹图谱研究[J].广东农业科学,2014,41(2):116-119 |
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白背叶根药材的HPLC指纹图谱研究 |
| Study on HPLC fingerprint of the root of Mallotus apelta |
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| DOI： |
| 中文关键词: 白背叶根 指纹图谱 3'-O-甲基鞣花酸-4-O-琢-L 吡喃鼠李糖苷 HPLC |
| 英文关键词: root of Mallotus apelta fingerprints 3'-O-methyl-ellagic acid-4-O-琢-L-rhamnopyranoside HPLC |
| 基金项目:广西科技攻关项目11107010-3-5 |
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| 摘要点击次数: 1342 |
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| 中文摘要: |
| 采用高效半制备液相色谱法分离，并以13C 谱法（13C-NMR）和1H 谱法（1H-NMR）确定结构；采用
HPLC 梯度洗脱进行色谱分离。对不同产地的10 批白背叶根药材指纹图谱的相似度进行比较分析。结果表明，分离
得到3'-O原甲基鞣花酸-4-O-琢-L 吡喃鼠李糖苷，纯度>98.50%（面积归一化法）；各批药材共建立了15 个共有峰，不
同产地的白背叶根药材指纹图谱相似度良好。说明高效半制备液相色谱法制备3'-O-甲基鞣花酸-4-O-琢-L 吡喃鼠
李糖苷的方法简单、高效，指纹图谱分析方法稳定性、精密度、重现性较好，用于白背叶根的质量评价切实可行。 |
| 英文摘要: |
| Semi-preparative HPLC was used to isolate and purify 3'-O-methyl-ellagic acid-4-O-琢-L-rhamnopyra鄄
noside from the root of Mallotus apelta. Its chemical structure was elucidated by 13C-NMR and 1H-NMR; 10 batches of the
roots of Mallotus apelta samples from different sources were analyzed by HPLC gradient elution method. The results showed
that the purity of 3'-O-methyl-ellagic acid-4-O-琢-L-rhamnopyranoside reached 98.50% by using area normalization
method, 15 common peaks were selected in chromatograms, all the common peaks were separated effectively, and 10
batches of the roots of Mallotus apelta fingerprint had good similarity. The results indicated that semi-preparative HPLC
was a simple, quick and efficient method to prepare 3'-O-methyl-ellagic acid-4-O-琢-L-rhamnopyranoside. Fingerprint
analytical method, being with good precision and repeatability, could be useful in the quality control of the root of Mallotus
apelta. |
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