| 颜雯1 李海涛2 向华1 王晓虎员.CRISPR-Cas 基因组改造技术研究进展[J].广东农业科学,2014,41(2):149-152 |
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CRISPR-Cas 基因组改造技术研究进展 |
| Research progress on the genome modificationtechnologies of CRISPR-Cas |
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| DOI： |
| 中文关键词: 规律成簇间隔短回文重复CRISPRs内切酶 同源重组机制 非同源末端连接机制脱靶效应 |
| 英文关键词: clustered regularly interspaced short palindromic repeats (CRISPRs) endonuclease homologous recombi
nation non-homologous end joining off-target effect |
| 基金项目:国家自然科学基金青年基金31201918广东省
自然科学基金博士启动项目s2012040007744广东省科技厅
农业攻关项目2012B020306010 |
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| 摘要点击次数: 1949 |
| 全文下载次数: 841 |
| 中文摘要: |
| CRISPRs-Cas 系统是一种细菌获得性免疫系统，为一段规律成簇间隔短回文重复，保护细菌和古生菌
免遭病毒、质粒等的入侵。这种免疫系统是由一种小RNA 和多结构域蛋白质/蛋白复合物构成, 其作用机理可能与真
核生物的RNA 干扰过程类似。这个系统已发展为一种高效、特异、操作简单易行的基因修饰工具，与TALEN、ZFN 相
比，CRISPR-Cas 系统的出现使得基因编辑变得更加有效和简易，具有更大的潜力。CRISPR/Cas 系统已成功应用到细
胞、干细胞、小鼠、斑马鱼及植物等多种生物，显示了其强大的基因编辑优点。综述了CRISPR-Cas 系统的技术原理及
其在生物学研究中的应用最新研究进展并探讨了该技术的发展前景。 |
| 英文摘要: |
| CRISPRs-Cas is the elucidation of the prokaryotic adaptive immune system and clustered regularly inter
spaced short palindromic repeats which protects bacteria and archaea against viruses or conjugative plasmids. The immuni
ty system consists of bacteria RNA molecules and versatile multi-domain proteins or protein complexes which may be simi
lar to the mechanism of RNA interference. CRISPR-Cas interference machines are utilized to develop into a genome edit
ing tools with efficiency, specialization and simplicity. Distinct from TALEN and ZFN, the CRISPR-Cas system has recent
ly emerged as a potentially facile and efficient alternative tool. Currently, researches have already modified the genome of
cell, IPS, mouse, zebra, fish and plant with this system that show powerful ability to edit gene. The technical principles of
CRISPR-Cas system and the latest progress on the application in biological study of CRISPR/Cas system was reviewed, and
the development future of the system was discussed |
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