| 樊永军1 赵艳玲1 闫伟2.贺兰山两株大型真菌物种分子
鉴定与系统地位分析[J].广东农业科学,2014,41(2):153-156 |
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贺兰山两株大型真菌物种分子
鉴定与系统地位分析 |
| Molecular identification and analysis of phylogenetic position fortwo wild fruiting body-forming fungi in Helan Mountain |
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| DOI： |
| 中文关键词: 野生真菌 分离 ITS 系统地位 |
| 英文关键词: wild fruiting body-forming fungi isolation ITS phylogenetic position |
| 基金项目:国家自然科学基金31260132，31060111内蒙
古自然科学基金2012MS0514 |
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| 摘要点击次数: 1436 |
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| 中文摘要: |
| 采用CTAB 法对采集于贺兰山地区的两株野生大型真菌子实体进行基因组DNA 提取，并利用真菌特
有引物ITS1F 和ITS4在PCR 基础上对其rDNA 的ITS 区段进行碱基序列测定并与GenBank 数据库中已知的相关
序列进行Blast 比对，结果表明，两株野生大型真菌分别属于鳞伞属Pholiota sp.和球盖菇属Stropharia sp.
GenBank 登录号分别为FJ596817.1 和JQ627593.1同时在UNITE Database(s)数据库中采用邻接法对两菌株进行系统
发育树的构建及遗传距离的计算，结果表明，一菌株与Pholiota tuberculosa (Pers.) Kuyper & Tjall.-Beuk
UDB015324另一菌株与Stropharia coronilla (Fr. ex Bull.) Qul. UDB015429 UDB015556）的遗传关系最近遗传
距离达到5.0。初步鉴定两株野生大型真菌为小瘤鳞伞和齿环球盖菇。 |
| 英文摘要: |
| The extraction of genomic DNA of two wild macrofungi obtained from Wulashan was carried out by CTAB
method. Then, on the basis of PCR, ITS regions of DNA of the macrofungi were amplifiedby using the special pairs of
primers ITS1F/ITS4 and sequenced. The sequences were compared with those registered in GenBank database, results of
which showed that two fungi belonged to the genus Pholiota sp. and Strophariaceae sp.. Genbank accession numbers of the
ITS gene was FJ596817.1 and JQ627593.1. Then the phylogenetic trees were constructed by neighbor-joining method and
genetic distance was analyzed in UNITE Database (s). The results showed that two wild fungi had a high genetic distance,
one with Pholiota tuberculosa (Pers.) Kuyper & Tjall.-Beuk (UDB015324), and the other with Stropharia coronilla (Fr. ex
Bull.) Qul.(UDB015429, UDB015556), which was up to 5.0. On the basis of the sequence analysis, two wild fruiting bodyforming
fungi were identified as Pholiota tuberculosa and Stropharia coronilla |
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