| 梅洪娟1，马瑞君2，庄东红1,2.指纹图谱技术及其在植物种质资源中的应用[J].广东农业科学,2014,41(3):159-164 |
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指纹图谱技术及其在植物种质资源中的应用 |
| Leaf morphology and genetic diversity of Aquilaria sinensisin Dongguan and its surrounding areas |
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| DOI： |
| 中文关键词: 指纹图谱 植物 种质资源 应用 |
| 英文关键词: Aquilaria sinensis ISSR genetic diversity genetic structure |
| 基金项目:广东省科技计划项目（2011B020304012）；广东省
高等学校科技创新重点项目（cxzd1131） |
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| 中文摘要: |
| 随着“指纹”的概念在诸多领域的引入，涌现出多种指纹图谱技术。将指纹图谱区分为以生物大分子作
为检测对象的生物指纹图谱和以植物中提取的化学成分为检测对象的化学指纹图谱。并对不同的指纹图谱技术的
概念、应用范围和差异等方面进行综述。同时对不同的指纹图谱技术在植物种质资源中的应用情况进行总结。 |
| 英文摘要: |
| The population genetic diversity and differentiation of Aquilaria sinensis were analyzed by using ISSR. A
total of 105 loci were generated by using 9 primers for 221 samples of 4 A. sinensis population. A toal of 104 loci were
polymorphic and the percentage of polymorphic bands (PPB%) was 99.05%. Observed number of alleles (Na) and effective
number of alleles (Ne) were 1.9905 and 1.5854 respectively. Effective number of alleles (H) and Shannon爷s information
index (I) were 0.3390 and 0.5054 respectively. These results indicated that there was a high level of genetic diversity in A .
sinensis at the species level. Total gene diversity (Ht) was 0.3236 among 4 populations of A. sinensis. Gene diversity within
population (Hs) is 0.2500. The Nei爷s coefficient differentiations (Gst) was 0.2274. The estimate of gene flow (Nm) was
1.6983. The results of UPGMA revealed that the samples of Dianbai population clustered with Dalingshan population
firstly, then clustered with Qingxi population. The samples of Guangxi population didn爷t cluster with the other populations
which showed that it had large genetic divergence form them. The results of UPGMA of individual samples revealed that
between the first and second generations of Dianbai population, there was larger divergence in the large-leaved subgroup
than that of small -leaved one. The small -leaved subgroup of Dalingshan population had close relation with the first
generation subgroup of Dianbai one. The large-leaved subgroup of Dalingshan population had close relation with the Qingxi
population. These results revealed that part of Dalingshan population might come from Qingxi population, and the rest of
Dalingshan population might have the same origins with Dianbai population. |
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