| 冯龙，许世杰，李园园，付官宝，吴昊伟，王倩，刘其根，曲宪成.黄鳝性逆转相关基因的表达及定位分析[J].广东农业科学,2014,41(5):190-193 |
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黄鳝性逆转相关基因的表达及定位分析 |
| Analysis on expression and localization of generelated to sex reversal of Monopterus albus |
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| DOI： |
| 中文关键词: 黄鳝 性逆转 荧光定量 原位杂交 |
| 英文关键词: Monopterus albus sex reversal quantitative PCR situ hybridization |
| 基金项目:农业部科技教育司项目(201003076) ；上海市重点
学科建设项目(S30701)；上海高校知识服务平台上海海洋大学水
产动物遗传育种中心项目（ZF1206） |
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| 中文摘要: |
| 为研究F25 基因与黄鳝性逆转关系，首先利用荧光定量PCR 技术对该基因在各期性腺组织中的表达量
进行分析，然后利用原位杂交技术合成cRNA 探针对F25 基因进行杂交定位分析。结果显示，F25 基因在II、III 期卵
巢中仅有微量表达，而在IV 卵巢中表达开始迅速增加，同时随着卵巢发育成熟、排卵、退化表达量也呈现不同程度
的升高，在精巢中该基因的表达量达到最大值；经定位分析发现，该基因在早期卵巢中基本不表达，在IV、V 期卵巢
中主要在体细胞中表达，而在精巢雄性生殖细胞中基本不存在F25 基因的表达，由此推测F25 基因可能在性逆转过
程中起到一定的作用，且主要作用于性腺组织中的体细胞。 |
| 英文摘要: |
| In order to research the relationship between gene F25 and Monopterus albus sex reversal, firstly, we used
quantitative PCR technology to analyze the gene expression in different gonadal tissues, then used situ hybridization technique to
synthesize cRNA probes and locate the F25 gene differential expression at different periods. The results showed that the gene
expressed little in ovary at stage II and III, and began to increase rapidly in ovary at stage IV, meanwhile, its expression also
showed different degrees with the ovarian maturation, ovulation, degradation, its expression reached maximum in the testis. By
positioning analysis, we found that the gene was not essential in the early ovary and mainly expressed in somatic cells in ovary at
stage of IV and V, whereas there was no F25 gene expression in germ cells of the testes, which indicated that F25 gene may play
a role in the process of sex reversal and affect on the somatic gonad tissues. |
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