| 罗焕明, 邓瑞云, 刘晓津, 邱道寿, 张蕾, 蔡时可.不同添加物及浓度对铁皮石斛组织培养的影响
x[J].广东农业科学,2014,41(7):30-32 |
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不同添加物及浓度对铁皮石斛组织培养的影响
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| Effects of different additives and concentrations onthe tissue culture of Dendrobium officinale |
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| DOI： |
| 中文关键词: 铁皮石斛 组织培养 添加物 |
| 英文关键词: Dendrobium officinale tissue culture additives |
| 基金项目:广东省产学研项目（2011B090400537，2012B0911
00168）；广东省社会发展重点项目（2011A030100002）；广东省良
种培育和引进项目（粤财农[2011]584 号，粤农计[2011]143 号） |
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| 中文摘要: |
| 以无菌播种取得的铁皮石斛无菌苗作为试验材料，研究1/2 MS 培养基中添加不同浓度的添加物对铁皮
石斛的增殖、生根的影响。结果表明；马铃薯汁和木瓜乳有利于诱导芽的增殖，其中又以1/2 MS+NAA 0.2 mg/L +蔗糖
30 g/L+马铃薯200 g/L 培养基最有利于诱导芽的增殖；香蕉乳有利于诱导生根，其中又以1/2 MS +NAA 0.5 mg/L+蔗
糖30 g/L +香蕉100 g/L 培养基最有利于诱导芽生根。 |
| 英文摘要: |
| The effects of different additives on proliferation and rooting culture of Dendrobium officinale were studied.
The results showed that: potato and papaya were conductive to bud proliferation, among which the 1/2 MS+NAA 0.2 mg/L+
sucrose 30 g/L+200 potato g/L medium was the best; banana promoted the rooting induction, in which 1/2 MS+NAA 0.5
mg/L+ sucrose 30 g/L+Banana 100 g/L medium was the best to induce the rooting of the shoots. |
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