| 于波，刘金梅，刘晓荣，廖飞雄，朱根发.两种抗生素对阿拉巴马花烛植株再生的影响[J].广东农业科学,2014,41(7):53-56 |
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两种抗生素对阿拉巴马花烛植株再生的影响 |
| Effect of two kinds of antibiotics on plant regenerationof Anthurium andraeanum cv. alabama |
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| DOI： |
| 中文关键词: 花烛 卡那霉素 潮霉素 外植体 植株再生 |
| 英文关键词: Anthurium andraeanum kanamycin hygromycin explant plant regeneration |
| 基金项目:广东省农业科学；；长基金（201217 |
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| 摘要点击次数: 1402 |
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| 中文摘要: |
| 花烛（Anthurium andraeanum Lind）是世界第二大热带盆栽花卉，其转基因育种具有巨大的应用前景。以
花烛为材料，研究不同浓度卡那霉素和潮霉素对阿拉巴马花烛植株再生的影响，结果表明；卡那霉素和潮霉素均能
促使外植体严重褐变死亡，同时，二者对愈伤组织和芽的诱导具有明显抑制作用；当以叶片或叶柄为转化受体时，选
择压以100 mg/L 卡那霉素或20 mg/L 潮霉素为宜；当以愈伤组织为转化受体时，选择压以150 mg/L 卡那霉素或40
mg/L 潮霉素为宜；两种抗生素对生根亦具有抑制作用，选择压则以150 mg/L 卡那霉素或40 mg/L 潮霉素为宜。 |
| 英文摘要: |
| Anthurium andraeanum is the second tropical pot flowers in the world, and its transgenic breeding has been
shown promising application. In this study, susceptibility of A. andraeanum cv. alabama to antibiotics (hygromycin and
kanamyein) was studied Results showed that kanamycin and hygromycin could cause seriously explant browning and dying,
and had significant inhibitory effect on the callus induction and bud differentiation. When the leaves and petioles were
used as receptors, the optimal selection pressure was 100 mg/L kanamycin or 20 mg/L hygromycin; When the calli were
used as receptors, the optimal selection pressure was 150 mg/L kanamycin or 40 mg/L hygromycin; The two antibiotics also
could inhibit rooting and the optimal selection pressure was 150 mg/L kanamycin or 40 mg/L hygromycin. |
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