文章摘要
乔培培1,2,陈丕茂1,冯雪1.高盐度海水氮磷营养盐保存技术研究[J].广东农业科学,2014,41(7):114-119
查看全文    HTML 高盐度海水氮磷营养盐保存技术研究
Study on preservation method of nitrogen andphosphorous in high salinity seawater
  
DOI:
中文关键词: 海水  盐度  氮磷营养盐  保存方法
英文关键词: seawater  salinity  nitrogen and phosphorus nutrient  preservation method
基金项目:国家科技支撑计划项目(2012BAD18B02);国家 公益性行业(农业)科研专项(201003068);中国水产科学研究: :级基本科研业务费专项(2012A0102)
作者单位
乔培培1,2,陈丕茂1,冯雪1 1.中国水产科学研究:南海水产研究所/农业部南海渔业资源环境科学观测实验站广东广州510300 2.上海海洋大学海洋科学学:上海201306 
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中文摘要:
      采用12 种不同方法保存盐度为32 的高盐度海水水样中的4 种氮磷营养盐(NH3-N、NO2-N、NO3-N、 PO4-P),使用FIAstar5000 营养盐流动注射分析仪对4 种营养盐在9 d保存期内参数值的稳定性进行研究。结果表 明,不同保存方法间存在差异,每种营养盐的最佳保存条件不同。9 d 试验期内,水样中NH3-N 的变化趋势较大, NH3-N 和PO4-P 的含量呈减小趋势,NO2-N、NO3-N 的含量略微上升。水样中加氯仿对氮营养盐(NH3-N、NO2-N、 NO3-N)的保存效果较好,加甲醛对磷营养盐(PO4-P)的保存效果较好。综合考虑试验结果的有效性和可操作性,得出 了4 种营养盐9 d内最适保存条件:常温下加5译氯仿,NH3-N 的保存效果能稳定在80%以上;在-20益加1译氯仿 时,NO3-N 的保存效果最好,9 d内稳定性都在95%以上;在-20益或4益下加5译或1译氯仿,NO2-N 在9 d内都能稳 定在95%以上;4益下加5译甲醛时,PO4-P 的保存效果最好。
英文摘要:
      In this paper, 12 different preservation methods were used to save 4 kinds of nitrogen and phosphorus nutrients (NH3-N, NO2-N, NO3-N, PO4-P) in high salinity sea water of salinity 32. We studied the parameter values' stability of 4 kinds of nutrients in 9 days storage using FIAstar5000 nutrient flow injection analyzer. The results showed that there were obvious differences among different preservation methods, the best preservation conditions of each nutrient was different. In 9 days experimental period, NH3-N in water samples had a large change tend, the NH4-N and PO4-P content presented a trend of decrease, while NO2-N and NO3-N content slightly rose. When chloroform was added into water samples, it was very good for the preservation of nitrogen nutrient (NH3-N, NO2-N and NO3-N); when formaldehyde was added, it was good for the preservation of phosphorus nutrient (PO4-P). Considered the effectiveness and operability of the experiment results, we obtained the optimal preservation condition of the 4 kinds of nutrient in 9 days. When 5译 chloroform was added into water samples, the stability of NH3-N could be controlled more than 80%; when 1 chloroform was added into water samples and the water samples were stored at -20, the stability of NO3-N could be over 95%; when 5译chloroform was added at -20 or 1译chloroform was added at 4益into water samples, the NO2-N could be more than 95%; when 5译formaldehyde was added at 4, it was the best method to save PO4-P.
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