| 韩义胜，严小微，唐清杰，邢福能.利用药用野生稻培育耐光氧化和
耐荫水稻新种质的研究[J].广东农业科学,2014,41(8):22-25 |
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利用药用野生稻培育耐光氧化和
耐荫水稻新种质的研究 |
| Study on breeding of new rice germplasm with photooxidationand shade tolerance by using Oryza officinalis |
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| DOI： |
| 中文关键词: 药用野生稻 耐光氧化 耐荫 育种 |
| 英文关键词: Oryza officinalis photooxidation tolerance shade tolerance breeding |
| 基金项目:海南省重点科技计划项目（ZDXM20120013） |
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| 中文摘要: |
| 将药用野生稻总DNA 通过穗茎注射法导入恢复系R225，创制耐荫、耐光氧化的水稻新种质。结果表明，
在T0和T1代幼苗期进行全天侯遮光处理15 d后，有90%~75%的秧苗黄化甚至枯萎；在T2和T3代孕穗期和灌浆结
实期，通过测定耐光氧化指数，最终筛选到耐荫、耐光氧化的3 个导入系；经SSR 标记检测，RM219 和RM5490 在导
入系中扩增出了与受体R225 不同的3 种带型，表明药用野生稻DNA 被成功导入受体R225 中，并据此推测，标记
RM219 和RM5490 很可能与耐荫或耐光氧化QTL 位点紧密连锁。 |
| 英文摘要: |
| Bred new rice germplasm with photooxidation and shade tolerance by ear-stem injecting the DNA of Oryza
officinalis into the restorer R225. About 90%-75% of the T0 and T1 generation seedlings turned yellow even died after fully
shaded day and night for 15 d. Finally, 3 plant lines with photooxidation and shade tolerance were screened by determining
the photooxidation-tolerance index in booting and filling stages in T2 and T3 generations. Three types of DNA ladders
different from R225 were amplified in transformed lines by SSR makers detecting. The results showed that the DNA of O.
officinalis was transformed into R225 successfully. Presumably, RM219 and RM5490 link closely to the QTLs of
photooxidation or shade tolerance in rice. |
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