| 沈城，杨筱珍，张金彪，洪宇航，何杰，王瑶，成永旭.千金对河蟹幼蟹生长的影响及其在体内的蓄积[J].广东农业科学,2014,41(8):166-170 |
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千金对河蟹幼蟹生长的影响及其在体内的蓄积 |
| Influence of Cyhalofop-butyl on growth and tissueaccumulation of juvenile Chinese mitten crab |
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| DOI： |
| 中文关键词: 千金 河蟹 生长 蓄积 |
| 英文关键词: Cyhalofop-butyl Chinese mitten crab growth accumulation |
| 基金项目:华东地区“稻-渔”耦合养殖技术研究与示范
（2012030 81-1）；上海市现代农业产业技术体系建设专项
（D2010100208)；国家自然科学基金（31272677）；中华绒螯蟹良种
培育与营养生理（2012AA10A409-5）；国家农业科技成果转化项
目（2012GB2C 00014 |
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| 摘要点击次数: 1097 |
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| 中文摘要: |
| 设定急性（96 h）和慢性（40 d）试验，以初步探讨千金对河蟹幼蟹生长的影响，用高效液相色谱测定千金
在河蟹体内的蓄积，并在急性试验中间隔12 h测定千金在水中96 h内的浓度变化，计算其降解率。结果表明，急性
试验中（千金浓度分别为11、5.5、2.8 滋g/mL），随着水体中千金浓度的升高，幼蟹死亡率呈上升趋势；在最高浓度（11
滋g/mL）组，河蟹幼蟹死亡率最高、为33.33%，与其他两组有显著性差异。慢性试验中（千金浓度分别为1.10、0.54、
0.22 滋g/mL），不同浓度千金浸泡对幼蟹的重量、死亡率、蜕壳率和平均蜕壳天数均未产生明显影响。在急性和慢性试
验中，千金在肝胰腺中含量均为最高，其最高蓄积量（在最高千金浓度组）为8.61 滋g/g，与其他各组间有显著性差异。
浓度分别为11、5.5、2.8 滋g/mL 的千金在水中降解速度很快，48 h内的降解率分别为83.91%、68.73%和53.09%，48 h
后降解率增加明显变缓。结合急性和慢性试验的结果表明，千金不会导致河蟹大量死亡，不会对河蟹的蜕壳与生长
产生明显影响，并且千金在河蟹体内并没有大量蓄积。 |
| 英文摘要: |
| Effect of Cyhalofop-butyl on the crab growth was preliminarily determined by acute (96 h) and chronic (40
d) experiments. During this process, the accumulation of Cyhalofop-butyl in juvenile Chinese mitten crab was detected by
HPLC, the change of Cyhalofop-butyl concentration in the water at 12 hours intervals up to 96 hours was observed and the
degradation rate of Cyhalofop-butyl was calculated. The results showed that during acute experiment, the concentrations of
Cyhalofop-butyl were 11 g/mL, 5.5g/mL and 2.8g/mL, respectively, the death rate of juvenile Chinese mitten crab
obviously increased with Cyhalofop -butyl concentration increasing, death rate was significantly higher in 11 滋g/mL
Cyhalofop -butyl treatment (33.33%) than in both 5.5g/mL and 2.8g/mL group. In the chronic experiment, the
concentrations of Cyhalofop-butyl were 1.1g/mL, 0.5g/mL and 0.2g/mL, no significant difference in weight, death
rate and average days of molt wasdetected. Both in the acute and chronic experiments, the Cyhalofop-butyl concentration in
hepatopancreas was the highest, followed by gill and muscle. The highest Cyhalofop-butyl concentration (in the highest
concentration treatment group) in hepatopancreas was 8.61 滋g/mL. The degradation speed of Cyhalofop-butyl with the
concentration of 11g/mL, 5.5 滋g/mL and 2.8 滋g/mL was respectively 83.91%, 68.73% and 53.09%. The degradation
rates of Cyhalofop-butyl significantly increased with the increase of time in 48 h, especially obvious in 24 h. In conclusion,
the results showed that the massive death of crabs weren't observed in Cyhalofop-butyl treatment. No obvious influence on
molt and growth of Chinese mitten crab by Cyhalofop -butyl treatment was found. Meanwhile, the accumulation of
Cyhalofop-butyl in juvenile crab was limited. |
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