| 赵爱奇，刘嘉荣，郝金凤，哈斯阿古拉.甜瓜β-氨基己糖苷酶基因家族的全基因组分析[J].广东农业科学,2014,41(8):185-188 |
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甜瓜β-氨基己糖苷酶基因家族的全基因组分析 |
| Genome-wide analysis of 茁-Hexosaminidasefamily genes in Cucumis melo |
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| DOI： |
| 中文关键词: 甜瓜 茁-氨基己糖苷酶 进化分析 生物信息学 |
| 英文关键词: Cucumis melo Hexosaminidase phylogenetic analysis bioinformatics |
| 基金项目:国家自然科学基金(31101559） |
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| 中文摘要: |
| 茁-氨基己糖苷酶(茁-Hexosaminidase, 茁-Hex, EC 3.2.1.52)是生物界广泛存在的一种糖苷水解酶。通过全
基因组信息鉴定甜瓜茁-氨基己糖苷酶基因家族，初步在甜瓜全基因组数据库中发现5 个茁-Hex 基因成员，采用
Pfam 等在线软件预测候选蛋白的结构域，去除2 个不完整的基因，最后将筛选出的3 个基因与拟南芥、青椒、番茄、
可可树、谷子、葡萄和黄瓜的相应基因编码的蛋白序列进行进化分析比对、建立系统发生树和保守基序的预测分析。
结果表明，进化树亚族成员中基序相同，并且在保守区序列高度相似；亚族之间基序差异也较小，保守区序列也具有
较高相似性。 |
| 英文摘要: |
| Hexosaminidase (-Hex, EC 3.2.1.52) is one of glycosidases, have been shown to be universally
distributed among most types of living organisms.Hexosaminidase family genes in Cucumis melo were genome-wide
analyzed and 5 members of Hex family genes were found in the melon genome database. With the analysis of protein
structure prediction software such as Pfam, we removed 2 incomplete genes and filtered out 3 genes in C. melo. These
protein sequences were analyzed by multi -sequence aligned, which were similar in Arabidopsis thaliana, Capsicum
annuum, Solanum lycopersicum, Theobroma cacao, Setaria italica, Vitis vinifera, C.sativus and C.melo. The structure
evolution relation and conserved motifs were researched by bioinformatics method. The results indicated that this gene
family was highly conservative with same motifs in the same 茁-Hex family. Each Hex family within phylogenesis tree
demonstrated a bit of differences and the major domain were conserved too. |
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