| 蒋丽花，傅明辉，彭进平
研项目（2012KJCX0043）.水葫芦肌动蛋白基因片段的克隆及序列分析[J].广东农业科学,2014,41(9):143- |
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水葫芦肌动蛋白基因片段的克隆及序列分析 |
| Cloning and sequence analysis of actin genefragment from Eichhornia crassipes |
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| DOI： |
| 中文关键词: 水葫芦 肌动蛋白基因 内标 |
| 英文关键词: Eichhornia crassipes actin gene internal control |
| 基金项目:国家自然科学基金（21177029）；广东省教育厅科 |
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| 中文摘要: |
| 利用植物肌动蛋白保守片段设计简并引物，通过RT-PCR 扩增出水葫芦肌动蛋白基因片段并连接到
pMD19-T 载体，转化E. coli，测序。序列分析结果表明，该序列与油棕、小“果蕉等植物的相似性程度达到85%以上，
分子进化分析水葫芦肌动蛋白基因序列与油棕、小“果蕉等单子叶植物亲缘关系较近，但并没有与某些植物的肌动
蛋白很好地聚成一类。本研究所克隆得到的水葫芦肌动蛋白基因序列能够为进一步研究水葫芦其他基因的表达提
供内标。 |
| 英文摘要: |
| Actin gene fragment from Eichhornia crassipes was amplified with degenerate primers designed according
to the conservative sequences of plant actins by RT-PCT method. It was linked to pMD19-T vector and transformed to E.
coli and then sequenced. Sequence analysis showed that the degree of similarity of this sequence and sequences from
Elaeis guineensis and Musa acuminate etc. reached above 85%. Phylogenetic analysis also revealed that the sequence from
E.crassipes was nearer relative to sequences form some monocot plants such as E.guineensis and M.acuminate etc., but it
could not be clustered into one single group with some plant sequences. The sequence of this study can provide an internal
control gene for further study of other gene expression of E.crassipes. |
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