| 赵艳1,2、阮云泽1、张晓波1.王草根际解磷菌的分离及解磷能力测定[J].广东农业科学,2014,41(10):59-62 |
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王草根际解磷菌的分离及解磷能力测定 |
| Isolation and phosphate solubilizing capacity determination ofrhizosphere phosphate-solubilizing microorganism in King grass |
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| DOI： |
| 中文关键词: 王草 根际 解磷菌 解磷能力 |
| 英文关键词: King grass rhizosphere phosphate-solubilizing microorganism phosphate dissolving capacity |
| 基金项目:海南省自然科学基金（312060）；海南大学青年基
金（qnjj1201） |
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| 中文摘要: |
| 选用蒙金娜有机培养基, 从王草根际分离出21 株具有溶磷能力的菌株, 利用溶磷圈法和钼锑抗比色法
对其解磷能力进行测定。结果显示、21 株菌株在蒙金娜有机磷培养基上均形成明显透明圈, 其培养48 h D/d 值在1.3~
7.0 之间, 72 h D/d 值在1.72~5.5 之间, 120 h D/d 值在1.33~4.67 之间；用钼锑抗比色法测定溶磷量为4.11~327.1 mg/
mL。经解磷能力分析, 菌株2-6尧2-7-1尧2-9尧4-4 具有较强的解磷能力、这些菌株在后续微生物菌肥研制中具有较大
潜力。 |
| 英文摘要: |
| Phosphate-solubilizing microorganisms were isolated from King grass rhizospheres using Mengjinna organic
culture media. The phosphate solubilizing capacities were investigated from 41 strains based on phosphate-solubilizing
circle and molybdenum blue method. The results indicated that 21 strains had ability to solubilize organic phosphate on
Mengjinna organic culture medium and 48 h D/d (diameter of phosphate-dissolving zone/diameter of bacterial clone) value
was from 1.3to 7.0; 72 h D/d value was from 1.72 to 5.5; 120 h D/d value was from 1.33 to 4.67. In liquid culture, the
available phosphate increment was from 4.11 to 327.1 mg/mL using molybdenum blue method by inorganic phosphatesolubilizing
microorganism. Strains 2-6, 2-7-1, 2-9 and 4-4 had higher ability of organic phosphate dissolving, showed
great potential for agricultural use in microbiological fertilizer. |
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