| 莫海萍1，李伯林1，苏玉卿2，李凤英1.罗汉果胚性愈伤组织的诱导及植株再生[J].广东农业科学,2014,41(12):33-36 |
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罗汉果胚性愈伤组织的诱导及植株再生 |
| Embryonic callus induction and plantletregeneration of Siraitia grosvenorii |
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| DOI： |
| 中文关键词: 罗汉果 子叶 胚性愈伤组织 不定芽 组织细胞学 |
| 英文关键词: Siraitia grosvenorii cotyledon embryonic callus adventitious buds cytohistology |
| 基金项目:教育部科学研究重点项目（00221）；广西师范大
学青年基金 |
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| 摘要点击次数: 1431 |
| 全文下载次数: 495 |
| 中文摘要: |
| 为建立罗汉果高频再生体系，以无菌萌发种子的子叶为外植体，对胚性愈伤组织的诱导及植株再生进
行研究。结果表明，不同6-BA 与2,4-D 或NAA 配比均能诱导罗汉果子叶形成愈伤组织，诱导率为100%，但NAA 比
2，4-D 更有利于罗汉果胚性愈伤组织的诱导。诱导子叶胚性愈伤组织的最适培养基为MS+6-BA 1.0 mg/L+NAA 0.5
mg/L，诱导率为76%。罗汉果胚性愈伤组织为淡黄色或黄色，结构松散，呈颗粒状。2.0 mg/L TDZ 能有效促进不定芽的
分化，分化率为83.3%。组织细胞学观察发现，子叶胚性愈伤组织通过器官发生途径再生植株。随着愈伤组织表面逐
渐转绿，其内部细胞首先分化形成维管组织，然后出现瘤状结构的外表面。在瘤状结构內靠近表层的部位有多个分
生细胞中心，分生细胞中心向外分化形成不定芽。 |
| 英文摘要: |
| The effects of different kinds and concentrations of hormones on embryonic callus induction and plantlet
regeneration were studied by using the aseptic seedling爷s cotyledon from the seeds of Siraitia grosvenorii as explants for
setting up the effective regeneration system. The results indicated that the callus induction rate reached 100% under the
different proportion of 6-BA and 2, 4-D or 6-BA and NAA. NAA was more suitable than 2, 4-D for embryonic callus
induction. The optimum medium for embryonic callus induction was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L, and the induction
rate reached 76%. The friable, granular, light yellow or yellow embryonic callus was selected. The optimum concentration
of TDZ for differentiation of adventitious buds was 2.0 mg/L, with the differentiation rate 83.3% . Histo -cytological
observation showed that regenerated plants were produced via organogenesis. The vascular tissue was formed firstly in inner
layer of embryonic callus when the surface of embryonic callus turned green. Many vessels were observed in the green
nodule. With further development, multiple meristamic centers could be found in area of near surface and they developed
adventitious buds finally. |
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