| 颜彦，胡伟.短柄草2C 型蛋白磷酸酶基因BdPP2C2
的克隆及表达分析[J].广东农业科学,2014,41(12):156-160 |
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短柄草2C 型蛋白磷酸酶基因BdPP2C2
的克隆及表达分析 |
| Cloning and expression analysis of BdPP2C2 genefrom Brachypodium distachyon |
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| DOI： |
| 中文关键词: 短柄草 PP2C 克隆 表达 |
| 英文关键词: Brachypodium distachyon PP2C cloning expression |
| 基金项目:2013 年海南省重大科技专项(ZDZX2013023-1-
16） |
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| 摘要点击次数: 1251 |
| 全文下载次数: 454 |
| 中文摘要: |
| 从短柄草中克隆获得一个2C 型蛋白磷酸酶基因，命名为BdPP2C2。序列分析结果表明，该基因ORF 的
长度为1 368 bp。进化分析结果表明，该基因所编码的蛋白与拟南芥AtPP2C56 的亲缘关系最近。实时荧光定量PCR
分析结果表明，该基因的表达显著受ABA、乙烯、双氧水3 种信号分子诱导，并且受高盐和低温胁迫诱导。这些结果
表明，BdPP2C2 是非生物逆境胁迫中的一个应答因子，并且可能受相关信号分子调控。 |
| 英文摘要: |
| A PP2C gene named BdPP2C2 was isolated from Brachypodium distachyon, and the result of sequence
analysis proved that the length of its ORF was 1 368 bp. Phylogenetic analysis revealed that BdPP2C2 had the closest
genetic relationship with AtPP2C56. Quantitative real-time polymerase chain reaction (qRT-PCR) assay reveale that the
expression level of BdPP2C2 was induced by ABA, ethylene, H2O2, NaCl and cold treatments. These results indicated that
BdPP2C2 was a response factor in the condition of abiotic stresses, and it might be regulated by relevant signaling
molecules. |
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