番石榴叶水提取物抗对虾白斑综合征
病毒有效成分初探

阴晓丽; 李卓佳; 管淑玉; 温为庚; 郭志勋

文章摘要

阴晓丽，李卓佳，管淑玉，温为庚，郭志勋.番石榴叶水提取物抗对虾白斑综合征病毒有效成分初探[J].广东农业科学,2014,41(13):87-93

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Preliminary study of the active ingredients againstWSSV in guava leaf water-extract

DOI：

中文关键词: 番石榴曰水提取物曰WSSV

英文关键词: Psidium guajava L water-extract white spot syndrome virus

基金项目:广东省海洋渔业科技推广专项（A201201E04）；广东省省级财政鱼病防治专项曰国家公益性行业（农业）科研专项（201103034）；国家现代农业（虾）产业技术体系建设专项（CARS-47）；广东省海洋经济创新发展区域示范专项（GD2012- A02-004）

作者	单位
阴晓丽，李卓佳，管淑玉，温为庚，郭志勋	中国水产科学研究院南海水产研究所/农业部水产品加工重点实验室/农业部渔业资源开发利用重点实验室；上海海洋大学水产与生命学院；广东药学院中药学院

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中文摘要:

利用有机萃取技术对番石榴叶水提取物进行了初步分离，得到石油醚相（S）、乙酸乙酯相（Y），正丁醇相（Z）和水相（W）4个组分。然后通过人工感染试验，确定了各组分抗对虾白斑综合征病毒（WSSV）的效果，并通过体外试验研究了番石榴叶水提取物及各组分对WSSV的影响。人工感染的结果显示：用乙酸乙酯相与正丁醇相这两种组分分别处理WSSV后，对虾在用WSSV人工感染后未出现死亡，而用石油醚组分处理WSSV后，对虾在用WSSV人工感染后第5d开始出现死亡，第12d全部死亡，表明番石榴叶中影响WSSV致病性的有效成分的极性较强，与乙酸乙酯和正丁醇相近。用番石榴叶水提取液及其各萃取组分体外处理WSSV不同时间后，试验组和对照组中的病毒拷贝数随时间推移略有降低，但在第120h结束时，各组中的病毒拷贝数还维持在较高水平；之后，将WSSV核酸暴露，并在体外用番石榴叶水提取物及其各萃取组分处理，荧光定量PCR结果显示：随着孵育时间的延长，各组病毒基因拷贝数无显著性变化。

英文摘要:

Guava leaf water -extract was separated preliminarily using organic solvent extraction method. Four components in petroleum ether phase (S), ethyl acetate phase (Y), n-butanol phase (Z) and water (W) were collected separately. The challenge test results showed that there were no deaths observed after shrimp infected by white spot syndrome virus (WSSV) incubated with the components in ethyl acetate phase (Y) or n-butanol phase (Z), respectively, but the shrimp started to die after infected by WSSV incubated with the components in petroleum ether phase (S) 5 d, and all died after 12 d. WSSV was incubated with guava leaf water-extract and its extraction content at different time and quantified qPCR. The results showed that the number of WSSV decreased with time passing whether in experimental group or in control group, but still remained at a high level at 120 h. The naked deoxyribonucleic acid (DNA) of WSSV was incubated with guava leaf water -extract and four extraction component respectively, no significantly differences of WSSV gene number were observed comparing to that at the experiment starting (P>0.05).

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