| 夏薇，李静，周丽霞1，杨耀东，肖勇，马子龙.椰子保守microRNA 预测和特征分析[J].广东农业科学,2014,41(14):130-135 |
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椰子保守microRNA 预测和特征分析 |
| Prediction and characterization of conserved microRNA in Cocos nucifera |
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| DOI： |
| 中文关键词: 椰子 保守miRNA 靶基因 |
| 英文关键词: Cocos nucifera conserved miRNA target gene |
| 基金项目:国家自然科学基金（31301358）；海南省自然科学基金（313058） |
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| 中文摘要: |
| 根据释放的椰子转录组序列和miRBase数据库中的microRNA（miRNA）序列信息，分析椰子中保守miRNA 及其相应靶基因的特征遥。根据miRBase数据库中植物miRNA序列，共预测了41个miRNA，分别属于22个miRNA家族。这些miRNA家族中13个家族在植物中非常保守，miRBase至少在19个物种中检测到，而miR902、miR2673和miR414这3个家族不保守，仅在1~2个物种中检测到。这些预测的miRNA的成熟序列长度大多为21个碱基（59.5%），而前体序列的长度大多为50~100个碱基（61.9%）。分析miR396家族前体序列时发现，成熟miRNA和成熟miRNA*（成熟miRNA形成发卡结构互补的序列）序列很保守，但是中间的序列变异很大。此外，预测miRNA的靶基因发现保守的miRNA家族的靶基因为一些重要的转录因子家族，与植物的生长发育相关，而不太保守的miRNA家族的靶基因编码与表型控制相关的蛋白。 |
| 英文摘要: |
| In this study, we predicted and characterized conserved microRNA (miRNA) and their target genes in Cocos nucifera based on sequence information of coconut transcriptome and miRNA in miRBase. Based on miRNA sequences in miRBase, 41 miRNAs were predicted, which were classified into 22 miRNA families. Moreover, thirteen of these miRNAfamilies were highly conserved in plants, which were detected in more than 19 species in miRBase, while miR902, miR2673 and miR414 family were not conserved and were detected in one to two species. Majority of mature miRNA sequences were 21 nucleotides in length (59.5%), while most of precursor of these miRNA were between 50 to 100 nucleotides. Comparison of precursor miRNAs of miR396 family showed that the region of mature miRNA and miRNA were highly conserved, but the loop region were highly variable. Meanwhile, predicted target genes of these miRNA families demonstrated that target genes of conversed miRNA family were mainly belong to gene family of important transcription factor related with plant growth and development, while gene targets of not conserved miRNA family encoded the proteins related to control plant phenotype. |
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