| 田双娥，蔡俊鹏.6种保护剂对蛭弧菌BDS01的保质作用研究[J].广东农业科学,2014,41(16):129-133 |
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6种保护剂对蛭弧菌BDS01的保质作用研究 |
| Effects of six kinds of protectants on shelf-life of Bdellovrio sp. BDS01 |
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| DOI： |
| 中文关键词: 蛭弧菌 保质期 Arrhenius方程 |
| 英文关键词: Bdellovibrio-and-like organisms shelf-life Arrhenius equation |
| 基金项目:国家自然科学基金（40776091） |
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| 摘要点击次数: 1617 |
| 全文下载次数: 629 |
| 中文摘要: |
| 研究了甘油尧蔗糖、棉子糖、葡萄糖、谷氨酸钠和内消旋肌醇6种保护剂分别对蛭弧菌BDS01的保质作用，并挑选出单一作用时保护效果较好的3种保护剂做正交试验，以优化出最佳保护剂配方。应用阿仑尼乌斯方程（Arrhenius方程）预测蛭弧菌BDS01 制剂浓度从1010PFU/mL降低2个log值的保质期，并对预测的保质期进行验证。结果表明，保护效果较好的3种保护剂依次是15%甘油、5%蔗糖、5%谷氨酸钠，优化得到的保护剂配方是17%甘油、5%蔗糖、7%谷氨酸钠；验证性试验结果表明，25℃的蛭弧菌BDS01制剂保质期与预测的结果一致，而4℃的蛭弧菌降低2个数量级的保质期为13周左右，略低于预测值。说明在Arrhenius方程基础上建立的预测模型可以用于预测蛭弧菌的保质期。 |
| 英文摘要: |
| In this paper, we studied effects of six kinds of protectants, including glycerol, sucrose, raffinose, dextrose,sodium glutamate, meso-inositol, on shelf-life of Bdellovibrio sp. BDS01, and then picked out three kinds of protectants with good effect to design orthogonal experiment for the best formula. With Arrhenius equation, the shelf-life of BDS01 microbial preparation was predicted when its concentration dropped down by 2 log, and then experiments were conducted to verify the predicting result. The results showed that the effects of 15% glycerol, 5% sucrose and 5% sodium glutamate were better than others. After testing, 17% glycerol, 5% sucrose, 7% sodium glutamate were optimized by orthogonal experiment.Results of constant temperature verification experiment showed that the real shelf-life of BDS01 at 25℃was constant withthe prediction result and the real shelf-life at 10℃and 4℃were both slightly lowed than the prediction results when BDS01 reduced 2 log. And the real shelf-life of BDS01 reducing 2 orders of magnitude at 4℃was about 13 weeks. |
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