| 何荆洲，卢家仕，卜朝阳，黄昌艳，闫海霞，王晓国.36 个蝴蝶兰品种遗传关系的ISSR 分析[J].广东农业科学,2014,41(21):140-143 |
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36 个蝴蝶兰品种遗传关系的ISSR 分析 |
| Genetic relationship analysis of 36 cultivars of Phalaenopsis based on ISSR molecular markers |
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| DOI： |
| 中文关键词: 蝴蝶兰 ISSR 亲缘关系 分子标记法辅助育种 |
| 英文关键词: Phalaenopsis ISSR genetic relationship molecular breeding |
| 基金项目:南宁科技攻关计划项目（20122075）；广西科技开发项目（桂科攻1222009，桂科能14123006-41）；广西农科院项目（农成转201402，桂农科2013YF04，桂农科2013YM42） |
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| 中文摘要: |
| 采用ISSR分子标记技术对36个蝴蝶兰栽培品种进行遗传多样性和亲缘关系的分析。结果表明，从100条ISSR引物筛选出11条引物共扩增出98条清晰的谱带，其中91条表现出多态性，多态比率为92.86%，品种间的相似系数在0.57~0.96 之间，表明供试品种间具有丰富的遗传多态性。并检测到4 条品种特异性条带，可用来鉴定供试蝴蝶兰种的3个品种。36个品种在遗传距离L=0.644处可分为7个组，3个最大的组分别代表了黄色系、白色系和红色系，而在同一色系的亲缘关系中又以唇瓣的颜色来聚类，可见聚类情况与花色特征比较一致。 |
| 英文摘要: |
| 36 Phalaenopsis cultivars’s the genetic diversities and the genetic relationships were analyzed by using molecular marker technique of inter-simple sequence repeat (ISSR). The results showed 11 primers selected out of 100 primers amplfied 98 reproducible DNA bands, of which 91 bands were demonstrated polymorphism, and the polymorphic rate was 92.86%. The similarity coefficient between the cultivars ranged 0.57-0.96, showing that there was rich genetic diversity among the cultivars. Moreover, 4 specific ISSR bands were obtained, which could be used to identify 3 varieties of Phalaenopsis tested. 36 varieties could be divided into 7 groups at the genetic distance of L=0.644. Among them, the 3 largest groups represented yellow, white and red, respectively, while in the same color relationships, the groups were separated by the lip color, which showed the result of clustering analysis was generally consistent with color. |
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