| 李莉1，刘瑛1，汪光义2，成家杨1.深圳近海海域产纤维素酶、脂肪酶和漆酶
真菌的筛选及酶学性质分析[J].广东农业科学,2014,41(24):142-146 |
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深圳近海海域产纤维素酶、脂肪酶和漆酶
真菌的筛选及酶学性质分析 |
| Screening and characterization of several extracellularenzymes producing fungi from Shenzhen coast |
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| DOI： |
| 中文关键词: 深圳海域 真菌 纤维素酶 脂肪酶 漆酶 |
| 英文关键词: Shenzhen coastal areas fungi cellulase lipase laccase |
| 基金项目:深圳市发展和改革委员会深圳市生物产业发展
专项资金扶持计划项目（835） |
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| 中文摘要: |
| 现有自深圳海域分离出的真菌31 株，其中包括22 株丝状真菌和9 株酵母菌。对各菌株进行纤维素酶、
脂肪酶和漆酶等胞外酶的定性筛选后，进行胞外酶定量分析。结果表明，在定性初筛的培养基生长5 d后，大多数菌
株呈现出2~3 种胞外酶活力的溶解圈，而9 个酵母菌菌株都没有检出漆酶活力。在定性测量的结果基础上，挑选出
产酶潜力大的2 株丝状真菌（PKU F16、PKU F18）和2 株酵母菌（PKU Y5、PKU Y8），进行3 种胞外酶的定量分析。在
各菌株培养4、6、9 d时，分别对3种胞外酶活进行测定，结果发现各菌株生产3 种胞外酶的定量分析结果与定性分
析结果基本吻合，且随着培养时间的增长，菌株的胞外酶活力呈现先增后减的趋势。在第6 天，F18 和Y8 产纤维素
酶活力分别高达1.13、1.31 U/min窑mL。Y8 的脂肪酶活力产量高达21.94 U/min窑mL。F18 和F16 产漆酶活力高达
14.61、10.50 U/min窑mL。 |
| 英文摘要: |
| In the present study, the abilities of 31 marine fungi for extracellular enzymes production were investigated,
including cellulase, lipase and laccase. These isolates, including 22 filamentous fungi and 9 yeasts, were isolated previously
from Shenzhen coastal areas and identified by using rDNA sequencing of ITS gene. Most of the strains were found to show
considerable extracellular enzymatic activities when assayed qualitatively on substrate plates after 5 d growth. The result
showed that none of the yeasts showed laccase activity. Additionally, the quantitative assay of 4 fungal isolates, PKU F16,
PKU F18, PKU Y5 and PKU Y8 demonstrated increase of enzymatic activity initially followed by a decrease on the 6th
day. And the highest activities of cellulase enzyme for F18 and Y8 were 1.13, 1.31 U/min mL, respectively; Y8 produced
lipase up to 21.94 U/minmL. F18 and F16 produced 14.61, 10.50 U/min mL of laccase respectively. |
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