| 刘小玉， 付登强， 陈良秋，等.油茶根际溶磷菌3-Y-08的筛选与鉴定[J].广东农业科学,2015,42(3):47-50 |
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油茶根际溶磷菌3-Y-08的筛选与鉴定 |
| Screening and identification of phosphate solubilizing bacteria in the rhizosphere of camellia |
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| DOI： |
| 中文关键词: 油茶 溶磷菌 筛选 鉴定 |
| 英文关键词: camellia phosphate solubilizing bacteria screening identification |
| 基金项目: |
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| 摘要点击次数: 1732 |
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| 中文摘要: |
| 采用传统的微生物分离培养法，对油茶根际溶磷菌进行分离，筛选出 6 株溶磷细菌。 利用透明圈法对油茶根际土壤中具有溶磷能力的细菌进行初筛；采用钼锑抗比色法测定发酵液的可溶性磷含量，对解磷菌株进行复筛，得出菌株 3-Y-08 的溶磷活性最强。 根据进行菌落形态特征、生理生化特征、16S rDNA 序列和系统发育分析等研究，初步鉴定菌株 3-Y-08 为巨大芽孢杆菌。 |
| 英文摘要: |
| Phosphate solubilizing bacteria were isolated from the rhizosphere of camellia袁and six strains were
screened by using traditional method of isolation and culture of microorganisms. By using the way of clear zone,
phosphate solubilizing bacteria from the rhizosphere soil were preliminarily isolated, and the high effective phosphate
solubilizing bacteria were further screened by using molybdenum-antimony anti-spectrophotometric method, strain 3-
Y -08 was the most active. The strains and 16S rDNA and phylogenetic tree were identified by studying the
morphology, cultural, physiological and biochemical properties. Thus the strain was preliminarily identified as
Bacillus megaterium. |
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