| 陈远凤， 李一平， 喻国辉，等.枯草芽胞杆菌TR21喷叶对粉蕉根系分泌酚酸的影响[J].广东农业科学,2015,42(3):56-61 |
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枯草芽胞杆菌TR21喷叶对粉蕉根系分泌酚酸的影响 |
| Effects of Bacillus subtilis strain TR21 spraying leaves on secretion of phenolic acids in drawf banana (Musa spp. ABB) root exudates |
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| DOI： |
| 中文关键词: 枯草芽胞杆菌 粉蕉 根系分泌物 酚酸 |
| 英文关键词: Bacillus subtilis drawf banana Musa spp. ABB root exudates phenolic acid |
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| 中文摘要: |
| 为探索枯草芽胞杆菌 TR21 喷叶对广粉 1 号和粉杂 1 号粉蕉根系酚酸分泌的影响，测定了不同处理不同时间根系分泌物中的酚酸含量，并利用高效液相色谱测定第 13 天的根系分泌物中的酚酸组成，以及甲醇溶解物对香蕉枯萎病病原菌 1 号和 4 号生理小种孢子萌发的影响。 结果显示，广粉 1 号 TR21 处理后 4d 和6d 根系酚酸分泌量高于对照，处理后 8、9、11、13d 低于对照；TR21 处理粉杂 1 号根系酚酸分泌量始终高于对照。广粉 1 号对照根系处理后 13d 分泌物中检测到阿魏酸、反式肉桂酸、芥子酸、对香豆酸、水杨酸和邻苯二甲酸，TR21 处理仅检测到阿魏酸和反式肉桂酸； 粉杂 1 号对照和 TR21 处理后 13d 的根系分泌物只检测到阿魏酸。 不同处理的甲醇溶解酚酸显著抑制香蕉枯萎病 1 号生理小种小型分生孢子萌发，粉杂 1 号的抑制效果比广粉 1 号明显，TR21 处理的粉杂 1 号抑制效果最显著。 甲醇溶解酚酸对 4 号生理小种的分生孢子也有显著抑制作用，但不同处理间差异不显著。TR21 喷叶可以改变粉蕉根系酚酸的分泌，但在抗感品种中引起的变化存在差异。 |
| 英文摘要: |
| In order to explore the phenolic acids secretion of drawf bananaMusa spp. ABB Guangfen No .1 and
Fenza No .1 seedlings after spraying by Bacillus subtilis strain TR21, the root exudates were collected at different
days after treated and the contents of phenolic acids were tested. The components of phenolic acids at the 13th day
were detected by High performance liquid chromatography (HPLC), and the effects of methanol dissolved phenolic
acids on the microconidia germination of Fusarium oxysporum f. sp. cubense race 1 and race 4 were tested. The
results showed that TR21 treatment increased root phenolic acids secretion of Guangfen No.1 at the 4th and 6th day,
but decreased it at the 8th, 9th, 11th and 13th day, TR21 treatment increased the phenolic acids secretion of
Fenza No.1 at all tested times. Seven kinds of free phenolic acids, including ferulic acid, trans-cinnamic acid, sinapic acid, p-coumaric acid, salicylic acid and alizaric acid were found in control Guangfen No.1 root exudates, but only ferulic acid and trans-cinnamic acid were found in TR21 treated. Ferulic acid was the only detected free phenolic acid in Fenza No.1 root exudates. The methanol dissolved phenolic acids reduced the race 1 microconidia germination significantly, especially those from TR21 treated Fenza No.1. Methanol dissolved phenolic acids also reduced race 4 microconidia germination, but there was no difference among different treatments. It was concluded that TR21 spraying leaves really changed the root phenolic acids secretion of drawf banana, but there were differences in resistant and susceptive variety. |
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