| 刘凤民， 张伟丽， 陈梓健，等.辐照柱花草M2代的农艺性状及分子水平的诱变效果分析[J].广东农业科学,2015,42(4):28-34 |
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辐照柱花草M2代的农艺性状及分子水平的诱变效果分析 |
| Agronomic traits of M2 generation of Stylosanthes inducedby 60Co -ray and mutagenic effect at molecular level |
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| DOI： |
| 中文关键词: 柱花草 60
Co酌 射线 农艺性状 SRAP |
| 英文关键词: stylosanthes 60Co-ray agronomic traits SRAP |
| 基金项目: |
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| 摘要点击次数: 1397 |
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| 中文摘要: |
| 选用 60
Co-酌 射线辐射柱花草种子，种植后经过连续 2 年的单株选择，统计 M2 代 23 个株系的株
高、重量、物候期、病害级数，并进行方差分析，并在 M2 代中选取 6 个株系和 2 个对照品种进行 SRAP 分析和
聚类分析。 结果表明， M2 代共有 10 个株系的单株干重大于 Nchp1（CK1），与 CK1 差异显著。 有 12 个品系的花
期早于 CK1，21 个品系的株高大于 CK1，13 个株系的病害级数小于 CK1；M2 代部分品系的 SRAP 分析和统计
结果表明，39 对引物扩增出 528 条带，平均每对引物扩增出 13 条带，多态性条带平均比率为 91.67豫，PIC 平均
值为 0.861。这些材料间的遗传相似系数(GS)的变异范围为 0.485~0.670，平均 GS 为 0.589。通过 UPGMA 分子系
统聚类法，以 GS=0.5700 为基线，热研 2 号与 Nchp1 及以 Nchp1 为亲本辐照筛选的 6 个后代株系分列在两个
不同的大类群，其中热研 2 号为第玉大类群，而 Nchp1 和其 6 个后代株系在第域大类群。 第域大类群中的 7 个
品系中以 GS=0.6250 为基线分为 3 个小类：Nchpzh004 与 Nchpzh01101 在第 1 小类，Nchpzh021、Nchpzh01102 与
nchp1 在第 2 小类，Nchpzh08 与 Nchpzh401 在第 3 小类。这些结果表明，无论 M2 农艺性状分析和分子水平检测
代都揭示了辐照后变异的多样性，辐照 M2 代柱花草品系（品种）间存在丰富的遗传多样性，同时也说明 SRAP
标记在柱花草基因组遗传多态性方面有较显著的检出效率。 |
| 英文摘要: |
| The seeds of stylosanthes were irradiated by 60Co-ray. Then the implementation of individualselection to stylosanthes progeny continued for two years. The plant height, weight per plant, phenology and diseaserate of anthracnose were determined on M2 generation, and these data were analyzed by variance analysis. Six linesof M2 generation and two control varieties of stylosanthes were analyzed by SRAP analysis and cluster analysis. Theresults showed that the weight per plant of 10 lines of M2 generation were significantly greater than that of Nchp1(CK1). Compared with CK1, flowering stage of plant was earlier in 12 lines, plant height of 21 lines were greater,disease rate of 13 lines were less. SRAP analysis revealed that 528 effective bands were selected from 39 pairs ofprimers, each pair of primer amplified 13 bands, the average percentage of polymorphic bands was 91.67% andaverage PIC was 0.861. The Neis genetic similaritycoefficient of all treatments ranged from 0.485 to0.670, and the average Neis coefficient was 0.589.Using GS 0.5700 as a baseline, these 8 stylosantheslines were classified into two major groups by UPGMAcluster analysis, named group and group. Group included Reyan 2, group ncluded nchp1 and 6 derivative progeny of nchp1. Using GS 0.6250 as a baseline, group was divided into three small classes,nchpzh004 and nchpzh01101 were in the first class, nchpzh021 nchpzh01102 and nchp1 were put in thesecond class, nchpzh08 and nchpzh401 belonged to the third class. These results demonstrated that geneticvariation had diversity in M2 generation of irradiated stylosanthes both at agronomic traits and molecular level.There was significant detection rate in examining genetic polymorphism at genome level by using SRAPmolecular marker on stylosanthes. |
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