| 彭邦星， 李 辉， 张 达，等.兴义鸭MEF2A基因的SNPs筛选及其生物信息学分析[J].广东农业科学,2015,42(4):86-92 |
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兴义鸭MEF2A基因的SNPs筛选及其生物信息学分析 |
| SNPs screening and bioinformatics analysis of MEF2A gene in Xingyi duck |
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| DOI： |
| 中文关键词: 兴义鸭 MEF2A 基因 SNPs 生物信息学 |
| 英文关键词: Xingyi duck MEF2A gene SNPs bioinformatics |
| 基金项目: |
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| 摘要点击次数: 1510 |
| 全文下载次数: 809 |
| 中文摘要: |
| 为了解鸭 MEF2A 基因的功能和结构以及其对鸭遗传效应的影响，采用混合 DNA 池、基因克隆、
PCR 产物直接测序和生物信息学相结合的方法，对兴义鸭的 MEF2A 基因进行研究。 结果并未发现 SNP 位点，
运用生物信息学方法对其蛋白质一级结构和二级结构进行分析，发现其包含 33 个 琢 螺旋、20 个 茁 折叠、44 个
无规则卷曲。 将获得的鸭的 MEF2A 蛋白序列与 NCBI 上提交的家鼠、牛、斑马鱼、猪、鸡的 MEF2A 序列相比对，
结果显示：鸭与鸡、鱼的亲缘关系相近，与家鼠的亲缘关系较远。 |
| 英文摘要: |
| In order to understand the structure and function of duck MEF2A gene and to understand their
genetic effect on duck, MEF2A gene was cloned by bioinformatics and PCR, and thepolymorphisms of exon of MEF2A gene in Xingyi duck were detected by direct sequencing of PCR products. The results showed that there wasno SNPs. Using bioinformatics method, the primary protein structure and secondary structures were analyzed, theresults showed that, the protein contained 33 -helics, 20 -sheets and 44 irregular coils. We got the duck MEF2A protein sequences and blasted MEF2A protein sequences to Mus musculus, Bos tarurs, zebra fish, Susscrofadomestica, Gallus domestiaus in NCBI, the results showed that duck had nearly genetic relationship with G. domestiaus and zebra fish, had far genetic relationship with M. musculus. |
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