| 龚 娜， 杨 镇， 李学龙，等.朝鲜淫羊藿组织培养关键技术研究[J].广东农业科学,2015,42(5):15-18 |
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朝鲜淫羊藿组织培养关键技术研究 |
| Key technology of tissue culture for tiller buds of Epimedium koreanum |
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| DOI： |
| 中文关键词: 朝鲜淫羊藿 组织培养 消毒 芽诱导 |
| 英文关键词: Epimedium koreanum Nakai tissue culture disinfection bud induction |
| 基金项目: |
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| 摘要点击次数: 1393 |
| 全文下载次数: 813 |
| 中文摘要: |
| 为建立朝鲜淫羊藿快繁体系提供依据，探讨朝鲜淫羊藿外植体消毒和芽诱导的影响因子，以朝鲜
淫羊藿分蘖芽为外植体，按不同消毒时间（2、4、2+2、6 min）、不同基本培养基（MS、WPM、N6）、不同浓度的生长
调节物质（6-BA、IBA、GA3），对芽的诱导与增殖条件进行了系统研究。结果表明，最好的消毒方法为75%乙醇
消毒30 s，再用0.1%HgCl2消毒2+2 min，外植体存活率最高，达87.5豫；芽诱导适宜的培养基为WPM+1.5 mg/L
6-BA+0.5 mg/L IBA+0.5 mg/L GA3，诱导率为81.67%；芽增殖的适宜培养基为WPM+1.5 mg/L 6-BA+0.5 mg/L
IBA。试验筛选出分蘖芽适宜的消毒方法及不定芽诱导、增殖的培养基，为建立朝鲜淫羊藿分蘖芽的组培快繁
体系奠定基础。 |
| 英文摘要: |
| In order to provide basis for establishing rapid propagation system of Epimedium koreanum Nakai,
this research discussed the influencing factors of explant disinfection and bud induction. Regarding the buds as
explants, with different sterilization time(2 min, 4 min, 2+2 min, 6 min), varieties of minimal mediums (MS, B5, WPM)
and different concentrations of plant growth regulators including 6-BA, IBA and GA3, the research systematically
studied the induction and propagation conditions for tiller buds. The suitable method for sterilizing bud was to
disinfect it with 75% ethanol for 30 s, and then treat it with 0.1% HgCl2 for (2+2) min, which could control the
survival rate above 87.5%. The optimal medium for bud induction was WPM+1.5 mg/L 6-BA+0.5 mg/L IBA+0.5 mg/
L GA3, with the induction rate of 81.67%. The propagation medium suitable for buds was WPM+1.5 mg/L 6-BA+0.5
mg/L IBA. The disinfection method suitable for tiller buds and the medium combination suitable for induction袁
propagation of adventitious buds were screened out, to lay the foundation for establishing the rapid cultivation system
for tiller buds of E. koreanum Nakai. |
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