| 王晓煌， 黄胜琴， 李 玲.4种抑菌剂在烟草开放式组织培养中的应用[J].广东农业科学,2015,42(5):58-62 |
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4种抑菌剂在烟草开放式组织培养中的应用 |
| Application of four kinds of bacteriostats in open tissue culture of tobacco |
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| DOI： |
| 中文关键词: 植物开放式组织培养 烟草 抑菌剂 |
| 英文关键词: open tissue culture of plant tobacco bacteriostats |
| 基金项目: |
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| 摘要点击次数: 1825 |
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| 中文摘要: |
| 为探究烟草开放式组织培养的适用抑菌剂配方‘在烟草组培培养基中分别加入不同浓度的卡那
霉素、头孢霉素、多菌灵和次氯酸钠 4 种抑菌剂‘研究其抑菌效果和烟草组织培养生长情况。 结果表明：培养基
添加 50 mg/L 头孢霉素为抑制细菌最佳浓度；添加多菌灵（50%可湿性粉剂）600 倍液为抑制真菌最佳浓度；仅
添加 0.1%次氯酸钠溶液既能同时抑制真菌和细菌‘且外植体分化状况最佳。 卡那霉素不适合做烟草开放式组
培抑菌剂。 |
| 英文摘要: |
| A proper bacteriostatic agent formula takes key function in the open tissue culture. In this paper, four
common bacteriostats (kanamycin, cephalosporin, carbendazim and sodium hypochlorite) were added to culture
mediums separately and the effects of them on tobacco growth status were observed. The results showed that bacterial
contamination would be less than 10% when 50 mg/L cephalosporin was added to the medium, while fungi
contamination would be less than 10% when carbendazim in 600-fold dilution (50%WP) was added. What爷s more,
bacterial and fungi contamination were both less than 10% when culture mediums contained only 1% sodium
hypochlorite, and the tobacco explant differentiation was the best. Kanamycin was not a appropriate bacteriostatic
agent for open tissue culture of tobacco. |
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