| 唐斌林，张 萌，何 红，等.两株海洋细菌AiL3和CⅢ-1混合发酵条件及对辣椒生长影响研究[J].广东农业科学,2015,42(7):-98 |
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两株海洋细菌AiL3和CⅢ-1混合发酵条件及对辣椒生长影响研究 |
| Mixed fermentation optimization of two strains of marine bactreia AiL3 and CⅢ-1 and their influences on pepper growth |
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| DOI： |
| 中文关键词: 海洋细菌 混合发酵 优化 辣椒 |
| 英文关键词: Stenocatantops splendens Xenocatantops brachycerus live feed entomophagous birds massrearing |
| 基金项目: |
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| 摘要点击次数: 1432 |
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| 中文摘要: |
| ：通过单因素和正交试验对海洋细菌AiL3和CⅢ-1两菌株混合发酵条件进行了优化，初步测定并比
较了混合发酵液与单菌株发酵液对辣椒的促生效果。结果表明，其最佳培养基为：酵母膏10.0…g/L、酵母粉5.0…g/
L、蔗糖8.0…g/L、木薯淀粉10.0…g/L、CaCl2 ·2H2O…0.2…g/L、NaCl…0.2…g/L、FeSO4 ·7…H2O…0.1…g/L、MnSO4 ·7…H2O…0.5…g/L；
最佳发酵条件为：初始pH值 7.0、装液量230…mL/500…mL、接种量4%、发酵温度28℃、转速180… r/min、发酵时间
52…h。优化后，两菌株的活菌数和芽孢量显著提高，活菌数达5.0×1010…
CFU/mL，芽孢量达 4.5×1010
…CFU/mL，芽孢
生成率达90%以上，比优化前的活菌数和芽孢量提高了100 倍以上。对盆栽辣椒幼苗进行灌根处理，两菌株的混
合发酵液相比单菌株发酵液，其株高、茎粗、鲜重等均有显著提高。 |
| 英文摘要: |
| The single factor and orthogonal experiments were adopted to optimize the mixed fermentation conditions
of marine bacteria AiL3 and CⅢ-1. Then the growth-promoting effects of AiL3 and CⅢ-1 were determined and
compared in pepper seedlings when the two strains fermentated seperately or mixed. The results showed that the medium
formula was consisted of yeast extract 10 g/L，yeast powder 5 g/L，sucrose 8 g/L，manioc starch 10 g/L，CaCl2·2H2O 0.2
g/L，NaCl 0.2 g/L，FeSO4·7 H2O 0.1 g/L，MnSO4·7 H2O 0.5 g/L. And the fermentation conditions were as follows the
initial pH at 7.0，the liquid volume 230 mL/500mL，inoculum 4%，temperature 28℃，speed 180 r/min，fementation time
52 h. Under the optimized fermentation medium and conditons，the amount of living cells and spores of the two marine
bacteria mixed fermentation increased by 100 times compared with the minimal medium. The production of living cells
was 5.0×1010 CFU/mL，the spores was 4.5×1010 CFU/mL，the rate of spores was more than 90%. The apparent indexes
including plant height，stem diameter，fresh weight of pepper plants were promoted significantly compared to blank group
when irrigated the mixed fermentation broths in pot experiment. |
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