| 祝天成，时 涛，李超萍，等.木薯细菌性枯萎病病原菌的RAPD分析[J].广东农业科学,2015,42(7):-121 |
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木薯细菌性枯萎病病原菌的RAPD分析 |
| RAPD analysis of Xanthomonas axonopodis pv. manihotis |
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| DOI： |
| 中文关键词: ：木薯黄单胞 遗传多样性 RAPD |
| 英文关键词: Xanthomonas axonopodis pv. manihotis genetic diversity RAPD |
| 基金项目: |
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| 摘要点击次数: 1415 |
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| 中文摘要: |
| ：为了研究木薯细菌性枯萎病病原菌种群间遗传、变异分化，选取来自各个地区的黄单胞病菌代表
菌株 45…个，利用多态性较好的…8 条引物对其基因组DNA 进行 PCR 扩增，并构建指纹图谱。利用统计分析软件
NTSYS 对样品的PCR 结果进行了UPGMA聚类分析，结果表明，供试菌株间遗传变异较大，菌株间存在丰富的遗
传多态性。经综合聚类分析，在75%…的相似水平上，可将供试菌株划分为7个类群。 |
| 英文摘要: |
| In order to study the genetic，variance and differentiation of Xanthomonas axonopodis pv.manihotis，45 strains from different areas and 8 primers with preferable polymorphism were selected，PCR amplification and fingerprints construction were finished. The cluster analysis of UPGMA was obtained based on the PCR outcomes，the big differentiation and abundant polymorphism existed among these tested strains. The integrated cluster analysis results showed that all the strains could be divided into 7 groups at the level of 75% similarity. |
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