| 刘观斌，王 淼，卢迈新，等.枯草芽孢杆菌在罗非鱼链球菌病综合防控中的应用研究[J].广东农业科学,2015,42(13):123-129 |
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枯草芽孢杆菌在罗非鱼链球菌病综合防控中的应用研究 |
| Application of Bacillus subtilis in comprehensive preventionand control of streptococcus disease of tilapia |
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| DOI： |
| 中文关键词: 枯草芽孢杆菌 罗非鱼 水质 免疫力 抗氧化能力 抗病力 |
| 英文关键词: Bacillus subtilis tilapia water
quality immune response antioxidant capacity resistance to disease |
| 基金项目: |
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| 中文摘要: |
| ：研究枯草芽孢杆菌（Bacillus subtilis）泼洒对罗非鱼抗无乳链球菌病的影响，为枯草芽孢杆菌在罗非
鱼链球菌病综合防控中的应用提供研究基础。在尼罗罗非鱼（Oreochromis niloticus）养殖池塘水体中泼洒不同浓度
（对照组：0；试验组1：1.0×104 CFU/mL；试验组2：1.0×105 CFU/mL）的枯草芽孢杆菌，监测水质指标、鱼体免疫
及抗氧化指标，并用无乳链球菌（Streptococcus agalactiae）对罗非鱼进行人工攻毒。结果发现，试验组1 水体的氨
氮、亚硝酸盐氮、总磷均低于对照组，试验组2 水体在养殖后14 d 和21 d 的总磷含量及养殖后28 d 的氨氮含量
高于对照组，其他时间均低于对照组；亚硝酸盐氮含量在养殖后21 d 及35 d 低于对照组，其他时间均高于对照
组。试验组罗非鱼血清的溶菌酶（LZM）、过氧化物酶（POD）、碱性磷酸酶（AKP）、总超氧化物歧化酶（T-SOD）
活性及总抗氧化能力（T-AOC）和对照组相比均有不同程度的升高。试验组1 及试验组2 的累计死亡率分别为
42.22%、24.44%，显著低于对照组的81.90%。在养殖水体中泼洒适当浓度的枯草芽孢杆菌可以改善水质，提高罗
非鱼机体的免疫力、抗氧化能力，进而提高罗非鱼抗无乳链球菌病的能力。 |
| 英文摘要: |
| To provide research basis of using probiotic for comprehensively preventing and controlling streptococcus
disease in tilapia aquaculture，effects of Bacillus subtilis as water addictive on disease resistance against Streptococcus
agalactiae in tilapia were studied. B. subtilis was added to the water tanks of tilapia at final concentration of 1.0×104 CFU/
mL for group 1 and 1.0×105 CFU/mL for group 2，with no probiotic added to control tanks. Water quality parameters，fish
immunity and antioxidant indicators were monitored. Fishes were challenged by S. agalactiae via intra-peritoneal injection at
the end of experiment. The results showed that the concentrations of ammonia，nitrite nitrogen，total phosphorus in water of
group 1 were lower than those of the control group，the indexes of group 2 were lower than those of the control group except
the total phosphorus contents after 14 d and 21 d，and the ammonia content after 28 d were higher than those of the control
group，and the nitrite nitrogen contents after 7 d，14 d and 28 d were higher than that of the control group. The activities of
serum lysozyme（LZM），peroxidase（POD），alkaline phosphatase（AKP），total superoxide dismutase（T-SOD）and total
antioxidant capacity（T-AOC）of tilapia increased in group 1 and group 2 compared to the control group in varying degrees.
The cumulative mortalities of group 1 and group 2 were
42.22%，24.44%，and significantly lower than that of the
control group（81.90%）. Appropriate concentration of B.
subtilis as water addictive in tilapia could improve water
quality and enhance immune of tilapia，thereby improve
disease resistance against S. agalactiae in tilapia. |
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