| 郝辉，孙斯文，宋金勇，等.响应面法优化黑曲霉（Aspergillus niger）SW06 产果胶酶发酵培养基条件[J].广东农业科学,2015,42(14):83-89 |
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响应面法优化黑曲霉（Aspergillus niger）SW06 产果胶酶发酵培养基条件 |
| Medium optimization of pectinase production by Aspergillusniger SW06 using response surface method |
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| DOI： |
| 中文关键词: 黑曲霉 果胶酶 发酵培养基 优化 |
| 英文关键词: Aspergillus niger pectinase fermentation medium optimization |
| 基金项目: |
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| 摘要点击次数: 1484 |
| 全文下载次数: 645 |
| 中文摘要: |
| 对果胶酶高产菌株黑曲霉（Aspergillus niger）SW06 进行液体发酵工艺条件优化。首先采用单因素
试验设计果胶酶发酵的最适温度和pH 值，并筛选了最适的碳源、氮源和无机盐。在此基础上，采用中心组合设
计进行回归分析及响应面分析，确定培养基的最佳组合。结果表明，果胶酶发酵的最适温度为28益，最适pH 值
为5，最适碳源、氮源分别为果糖、蛋白胨。回归分析得到的优化发酵条件为：100 mL 培养基中含果糖4.89 g、蛋
白胨0.50 g、果胶0.60 g。在最优条件下发酵，果胶酶活最高可达4 175.65 U/mL。 |
| 英文摘要: |
| Using central composite design and response surface methodology, the fermentation condition of
pectinase producing strain Aspergillus niger SW06 was optimized. Firstly, the optimum temperature and pH for
pectinase production was optimized and the optimal carbon source, nitrogen source and inorganic salts were selected
by single factor method. Based on the single factor test, a central composite design was used for regression analysis to
determine the optimal culture conditions. The results showed that the optimal temperature and pH were 28益and 5,
respectively. The optimal carbon and nitrogen sources were fructose and peptone, respectively. The optimal
fermentation conditions were as follows: 4.89 g/100mL fructose, 0.50 g/100mL peptone, 0.60 g/100mL pectin. Under
the optimal fermentation conditions, the pectinase activity was up to 4 175.65 U/mL |
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