| 徐小萍，谢燕萍，慕 尧，钟春水，陈芳兰，张梓浩，陈裕坤，郭容芳，林玉玲，赖钟雄.HPLC 法测定LED 不同光质下迷迭香
愈伤组织中鼠尾草酸含量[J].广东农业科学,2015,42(15):77-81 |
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HPLC 法测定LED 不同光质下迷迭香
愈伤组织中鼠尾草酸含量 |
| Carnosic acid content determination in Rosmarinus officinalis L.callus under different qualities of LED light by HPLC |
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| DOI： |
| 中文关键词: 迷迭香 愈伤组织 LED 光质 鼠尾草酸 HPLC |
| 英文关键词: Rosmarinus officinalis L. callus LED light quality carnosic acid HPLC |
| 基金项目:国家级大学生创新创业训练项目
（111ZC1401）；福建农林大学高水平大学建设项目（闽
农林大规划［2014］12 号） |
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| 中文摘要: |
| 以迷迭香（Rosmarinus officinalis L.）愈伤组织为材料，采用高效液相色谱法（HPLC）测定了不
同LED 光质下迷迭香愈伤组织中鼠尾草酸的含量。HPLC 法测定鼠尾草酸的色谱条件为：C18 色谱柱，柱温
30℃；流动相为乙腈∶0.1% 磷酸= 55∶45（V/V），流速1.5 ml/min，紫外检测波长210 nm，等度洗脱，时间
30 min。结果表明：与黑暗和日光灯白光下的愈伤组织对照，LED 黄光下愈伤组织生长最好，LED 白光下生长
较好，LED 蓝光下生长最差；不同LED 光质下迷迭香愈伤组织中鼠尾草酸含量从高到低依次为：LED 白光>
日光灯白光> LED 蓝光>LED 红光>LED 绿光>LED 黄光；LED 白光下迷迭香愈伤组织鼠尾草酸含量最高，同
时较利于愈伤组织生长。 |
| 英文摘要: |
| The callus of rosemary（Rosmarinus officinalis L.）was used as materials，the content of carnosic
acid in callus under different LED light was detected by High Performance Liquid Chromatography（HPLC）. The
C18 chromatographic column was utilized for analysis. The column was eluted at 30℃ with isocratic elution of 55%
acetonitrile and 45% solution（0.1% phosphoric acid）for 30 min at a flow rate of 1.5 mL/min. The elunet was monitored
at 280 nm by using UV spectra. The results showed that compared with callus under dark and white fluorescent lamp，the
callus cultured under LED yellow grew best， those under LED white also grew well，while those under LED blue grew
worst. The order of carnosic acid content of rosemary callus under different LED lights was LED white > white fluorescent
lamp > LED blue > LED red > LED green > LED yellow. Under LED white，the content of carnosic acid in callus was
the highest and the callus also grew well. |
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