| 韩 倩，崔堂兵.芽孢杆菌C 产亚硝酸还原酶的发酵
培养基优化及酶学性质研究[J].广东农业科学,2015,42(16):71-70 |
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芽孢杆菌C 产亚硝酸还原酶的发酵
培养基优化及酶学性质研究 |
| Fermentation medium optimization and enzyme propertiesof nitrite reductase produced by Bacillus sp. C |
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| DOI： |
| 中文关键词: 亚硝酸还原酶 发酵培养基优化 酶学性质 响应面分析 |
| 英文关键词: nitrite reductase fermentation medium optimization enzymatic properties response surface analysis |
| 基金项目:广东省自然科学基金（S2013010013162）
作者简介：韩倩（ 1 9 8 9 - ），女，在读硕士生，
E-mail：952324365@qq.com |
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| 中文摘要: |
| 通过单因素试验和响应面分析对亚硝酸还原酶产生菌C 的发酵条件进行优化，获得最佳发酵
培养基为：葡萄糖2.26%，蛋白胨0.2%，NH4Cl 0.12%，K2HPO4·H2O 0.2%，MgSO4 0.075%，KCl 0.1%，FeSO4
0.004%，NaNO2 0.2%，在此条件下，酶活达到317.82 U/mL，比优化前的190.26 U/mL 提高了67.05%。产生的
亚硝酸还原酶的酶学性质表明，该酶被Mn2+、Mg2+、Na+ 激活，受Fe2+、EDTA 抑制；最适温度和pH 值分别为
30℃和7.4；当温度低于50℃、pH 值在7.0~8.0 时，酶能保持较高的活性。与现有文献报道的筛选细菌相比
较，该菌株亚硝酸还原酶产量处于较高水平。 |
| 英文摘要: |
| The fermentation conditions of nitrite reductase produced by Bacillus sp. C were optimized by single
factor experiment and response surface analysis. The optimal fermentation conditions were as follows：2.26% glucose，
0.2% peptone，NH4Cl 0.12%，K2HPO4·H2O 0.2%，MgSO4 0.075%，KCl 0.1%，FeSO4 0.004%，NaNO2 0.2%. In
these conditions，the enzyme activity reached 317.82 U/mL，increased by 67.05% compared with the activity before
optimization with 190.26 U/mL. The results showed that the nitrite reductase was activated by Mn2+，Mg2+，Na+ and
inhibited by Fe2+ and EDTA；the optimal temperature was 30℃，the optimal pH was 7.4；the enzyme preserved higher
activity at the temperature below 50℃ and pH 7.0-8.0. Compared with the existing literature，the yield of nitrite
reductase produced by Bacillus sp. C was higher |
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