| 康萍芝 1 ， 吴秀红 2 ， 张丽荣 1.放线菌代谢产物对蔬菜土传病原真菌
的抑菌活性及毒力测定[J].广东农业科学,2015,42(17):72-75 |
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放线菌代谢产物对蔬菜土传病原真菌
的抑菌活性及毒力测定 |
| Antimicrobial activity and virulence of actinomycetes metabolites against vegetablesoil borne pathogenic fungi |
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| DOI： |
| 中文关键词: 土壤放线菌 代谢产物 抑菌活性 毒力 |
| 英文关键词: soil actinomycetes metabolites antimicrobial activity virulence |
| 基金项目:宁夏自然科学基金（NZ13110） |
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| 中文摘要: |
| 采用平板对峙培养法、 菌丝生长速率法等研究方法， 测定了土壤放线菌代谢产物对蔬菜土传病原
真菌的抑菌活性和毒力。 结果表明： 从宁夏 10 多个市县采集设施蔬菜等多种作物根部和不同植被的 122 份土
壤样品中分离获得 483 个放线菌株， 并进一步纯化、 观测和确定了生长速度较快， 长势良好的放线菌菌株 212
株， 通过拮抗作用、 抑菌活性和毒力测定， 选择出了对蔬菜土传病害多种病原真菌具有显著抑制作用的生防放
线菌优良菌株 3 种， 为生物农药的开发提供良好菌源。 |
| 英文摘要: |
| By means of plate confrontation culture and mycelium growth rate， the antimicrobial activity and
virulence of metabolities of soil actinomycetes against vetetable soil borne pathogenic fungi were tested . The results
showed that 483 actinomycete strains were isolated from 122 soil samples of protected vegetable， many other crop
roots and different vegetatation from more than 10 cities and counties in Ningxia， and further purified， observed and
determined 212 strains of actinomycetes with faster growth rate and we well growth. Through determination of the
antagonism， antibacterial activity and virulence， 3 strains of biocontrol actinomyces which had significant inhibitory
effect on many pathogenic fungi of vegetable soil borne diseases were screened out in order to provide good source of
actinomycete strains for the development of biological pesticide. |
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