| 徐 磊，胡小文，姚艳丽，邢淑莲，刘 洋.割手密谷胱甘肽硫转移酶基因SsGST
的克隆与生物信息学分析[J].广东农业科学,2015,42(18):14-19 |
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割手密谷胱甘肽硫转移酶基因SsGST
的克隆与生物信息学分析 |
| Cloning and bioinformatics analysis of glutathioneS-transferase gene SsGST in Saccharum spontaneum L. |
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| DOI： |
| 中文关键词: 割手密 GST 基因 电子克隆 RT-PCR 生物信息学 |
| 英文关键词: Saccharum spontaneum L. GST gene in silico cloning RT-PCR bioinformatics |
| 基金项目:中央级公益性科研院所基本科研业务费专
项（ITBB120504）；中国热带农业科学院湛江实验站科研
启动专项（zjky201504） |
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| 摘要点击次数: 1458 |
| 全文下载次数: 586 |
| 中文摘要: |
| 为克隆割手密谷胱甘肽硫转移酶基因，采用电子克隆和RT-PCR 技术获得了1 个割手密GST 基
因，命名为SsGST，并对其进行生物信息学分析。序列分析表明，割手密SsGST 基因cDNA 全长702 bp，编码
224 个氨基酸，蛋白分子式为C1119H1756N300O327S5，预测蛋白质分子量为24.8 ku，等电点为6.21。蛋白疏水性分
析表明SsGST 蛋白为亲水性蛋白。保守结构域预测表明SsGST 蛋白具有GST-N 和GST-C 结构域。SsGST 蛋白
序列与玉米、高粱、谷子和甘蔗等植物的GST 蛋白序列相似性较高，系统进化树分析表明，SsGST 蛋白与玉米
GST 蛋白亲缘关系最近。 |
| 英文摘要: |
| To clone glutathione S-transferase gene（GST）of Saccharum spontaneum L. In this study，a
GST gene from S. spontaneum L. was cloned by in silico cloning and RT-PCR，named as SsGST，characteristics
of SsGST gene were analyzed using bioinformatics tools. Sequence analysis showed that the full length
cDNA of SsGST was 702 bp，which encoded 224 amino acids. The predicted protein molecular formula was
C1119H1756N300O327S5，whose molecular mass was 24.8 ku and isoelectric point was 6.21. Hydrophobic analysis
showed that SsGST protein was a hydrophilic protein. Conserved domain prediction indicated that SsGST protein
had GST-N domain and GST-C domain. SsGST protein sequence had higher similarities with the GST protein
sequence of maize，sorghum，foxtail millet and sugarcane and so on. Phylogenetic analysis showed that SsGST
protein was close to GST protein of maize. |
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