| 吴颜洲1，梁芳莹2，黄江华2.金心也门铁叶斑病病原菌鉴定[J].广东农业科学,2015,42(20):68-71 |
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金心也门铁叶斑病病原菌鉴定 |
| Identification of pathogen of leaf spotdisease in Dracaena arboreavar |
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| DOI： |
| 中文关键词: 金心也门铁 叶斑病 尖孢镰刀菌 鉴定 |
| 英文关键词: Dracaena arboreavar leaf spot disease Fusarium oxysporum identification |
| 基金项目:广东省科技厅项目（粤科规划字［2013］
137 号） |
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| 摘要点击次数: 1221 |
| 全文下载次数: 465 |
| 中文摘要: |
| 通过形态学观察、诱导产孢、致病力测定和分子生物学研究，对引起广州地区金心也门铁叶斑病
的病原菌进行鉴定，确定其为尖孢镰刀菌（Fusarium oxysporum Schl.）。病原菌在金心也门铁植株上接种后表
现近圆形或椭圆形水渍状病斑等症状，与田间自然发病植株一致；在PDA 培养基上菌落初期为白色，后转为
紫红色，大型分生孢子有3~5 个分隔，呈纺锤形至镰刀形，基部有脚胞；供试菌株的rDNA-ITS 与GenBank 中
已有的Fusarium oxysporum，登录号为LN828176.1、LN8-28174.1、KM282627.1 的序列相似性均高达100%。 |
| 英文摘要: |
| The pathogen causing leaf spot on Dracaena arboreavar in Guangzhou was identified as Fusarium
oxysporum Schl. The leaves showed symptoms of leaf spot，nearly round or oval shaped lesion after inoculating with the
pathogen，which were consistent with the symptoms of the natural infected plants. All isolates colonies showed white at
the early stage，then became red to purple，macroconidium 3-5 separated，fusiform to falcate，basal foot cell. Identity
of isolates were confirmed by analysis of rDNA-ITS sequences with primers ITS1-ITS4. BLAST searches showed that it
had 100% identity with Fusarium oxysporum isolates from Genbank，Genbank accession number were LN828176.1，
LN8-28174.1，KM282627.1 |
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