| 王土连，张 晟，黄绮薇，柯德森.家蝇蝇蛆耐高温抗氧化多肽的筛选及性质分析[J].广东农业科学,2015,42(21):145-149 |
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家蝇蝇蛆耐高温抗氧化多肽的筛选及性质分析 |
| Screening and characteristics of antioxidant peptideswith thermostability from housefly maggot |
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| DOI： |
| 中文关键词: 蝇蛆 抗氧化多肽 耐高温 NBT 法4 |
| 英文关键词: maggot antioxidant peptides thermostability nibroble tetrazolium（NBT） |
| 基金项目:广东省自然科学基金（ 2 0 1 4 A 0 3 0
3 1 3 5 3 1 ）；广东省专业综合改革试点项目（ 生物工
程，粤教高函［2013］113 号）；广州市科技计划项目
（201510010108）；广州大学国家级大学生创业训练项目
（2015） |
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| 中文摘要: |
| ：将家蝇（Musca domestica）蝇蛆进行不同高温的水浴处理，测量蝇蛆抗氧化性的变化。结果显示，
50℃处理抗氧化活力变化不显著，80、100℃处理短时间内出现显著下降，但残留的抗氧化活力在随后的高温
中保持稳定，提示家蝇蝇蛆存在耐高温抗氧化物质。将蝇蛆提取液在100℃处理不同时间以筛选耐高温抗氧化
物质，结果表明，经100℃处理5 min 筛选到的抗氧化物质在50、80、100℃保温5 h，甚至在120℃超高温条件
下保温30 min，其抗氧化活力均保持稳定。SDS-PAGE 电泳结合三氯乙酸沉淀反应结果证明，该物质为分子量
约为30 ku 的多肽。 |
| 英文摘要: |
| The maggots of Musca domestica were bathed in different temperatures of water，and the change of
antioxidant activity was measured. The results showed that antioxidant activity had no significant change bathing at
50℃，while that at 80℃ and 100℃ had significant decline in a short time，then remained stable，which suggested
that housefly bots have high temperature resistant antioxidant material. The maggot extracts were bathed at 100℃ for
different time，and the antioxidant activity was measured to screen antioxidant material with thermostability. The results
showed that the residual antioxidant substances after treatment at 100℃ for 5 min，could remain stable at 50℃，80℃，
100℃ for 5 h，even under the condition of 120℃ for 30 min. Comprehensive analysis combined with SDS-PAGE
electrophoresis and trichloroacetic acid precipitation showed that the antioxidant material was a 30 ku peptide. |
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