| 朱红根1，王菁菁2，胡蓉花1，刘琼光2，罗建军2.江西烤烟棒孢霉叶斑病病原菌鉴定[J].广东农业科学,2015,42(22):62-66 |
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江西烤烟棒孢霉叶斑病病原菌鉴定 |
| Identification of pathogen causing corynesporaleaf spot of tobacco in Jiangxi |
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| DOI： |
| 中文关键词: 烤烟 棒孢霉 叶斑病 鉴定 江西 |
| 英文关键词: flue－cured tobacco Corynespora cassiicola corynespora leaf spot identification Jiangxi |
| 基金项目:中国烟草总公司江西省公司科技项目（赣
烟司［2015］7 号）；江西省吉安市烟草专卖局（公司）
科技项目（吉烟科［2014］3 号） |
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| 中文摘要: |
| 为了鉴定江西省吉安地区一种新的烤烟叶斑病病原菌，研究了该菌的致病性、形态特征以及温度
对其生物学特征的影响，分析了其核糖体DNA－ITS 序列同源性。结果表明，该病原菌属于棒孢属真菌，同源
性分析显示，该菌与 GenBank 中多主棒孢霉 ITS 序列的同源性达99% 以上，确认该病原菌为多主棒孢霉
〔Corynespora cassiicola（Berk. ＆ Curt.）Wei〕；生物学特性测定结果表明，菌丝在低于9℃、高于39℃条件下
不生长，生长最适温度为26.5℃；最适于产孢温度为29℃，而分生孢子萌发最适温度为26.5℃。烤烟棒孢霉叶
斑病在江西属首次报道。 |
| 英文摘要: |
| In order to identify the pathogen of flue－cured tobacco corynespora leaf spot disease in Ji’an，Jiangxi
Province，the fungi was isolated from diseased tobacco leaves. Based on the pathogenicity，morphological characteristics
and the sequence of ribosomal DNA －ITS，the pathogen was identified as Corynespora cassiicola，and the homology
analysis showed the sequence of ribosomal DNA －ITS isolated from the strains had homology of more than 99% with
Corynespora cassiicola published in GenBank. Finally，the pathogen was indicated as Corynespora cassiicola（Berk. ＆
Curt.）Wei. The results of biological characteristics showed that fungus couldn’t survive below 9℃ or above 39℃，grew
best at 26.5℃. It produced the most spores at 29℃，and the conidia germination was the most at 26.5℃. The disease is
the first report in Jiangxi，China. |
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