| 丰 锋，叶银芳，叶春海.温度对香蕉不定芽生长及抗氧化酶活性的影响[J].广东农业科学,2016,43(7):25-30 |
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温度对香蕉不定芽生长及抗氧化酶活性的影响 |
| Effects of different temperatures on growth and root antioxidant enzyme activities of banana adventitious bud |
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| DOI：10.16768/j.issn.1004-874X.2016.07.005 |
| 中文关键词: 香蕉试管苗 抗氧化酶 根系活力 游离脯氨酸 丙二醛 |
| 英文关键词: banana plantlet antioxidant enzyme root activity free proline MDA |
| 基金项目:国家级大学生校外实践教学基地项目；广东省高等学校本科特色专业、专业综合改革、试点专业项
目；广东省重大科技专项（2012A020200009，2013B020201004）；广东省现代农业产业技术体系建设专项（0909354） |
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| 中文摘要: |
| 为探明温度对香蕉不定芽生长及根系活力的影响，以巴西香蕉（Musa paradisiaca）为材料，在人工气候箱条件下研究温度对香蕉不定芽生长及抗氧化酶活性的影响。结果表明：SOD 活性呈现上升- 下降波动变化，POD 活性先上升，培养15～20 d 达到最高后下降，培养45 d 后又缓慢上升，CAT 活性整体先下降，培养45 d 后上升；在30℃以内，随温度升高，根系活力逐渐增加，培养20 d 后游离脯氨酸含量随温度升高而上升，培养50 d 开始下降，MDA 含量先逐渐下降后上升，培养20 d 和45 d 较低；30℃最适宜香蕉不定芽生长，此条件下培养25 d 根系活力达最高（11.92 mg /g·h），游离脯氨酸（58.76 μg/g）和MDA含量（7.15 nmol/g）都较低，根茎叶生长旺盛（苗高5.02 cm、叶数3.64 片、根数4.63 条、根长4.91 cm），是最适宜移栽的时间；培养45 d 根系活力为2.33 mg/g·h，游离脯氨酸（94.83 μg/g）和MDA 含量（9.83nmol/g）都较低，根茎叶生长旺盛（苗高6.15 cm、叶数4.97 片、根数6.01 条、根长6.06 cm），是次适宜移栽的时间。30℃最适宜香蕉不定芽的生长，培养25 d 是最适宜移栽的时间。 |
| 英文摘要: |
| To clear the effects of temperature on the growth of banana adventitious bud and its root activity，
taking‘ Brazil’ banana（ Musa paradisiaca） as materials in the artificial climate chamber conditions. The results
showed that SOD activity first increased and then decreased. POD activity first elevated and reached the highest in
the 15-20 d，and then declined and slowly improved after 45 d. CAT activity first fell and increased after 45 d. At
30℃，with the increase of temperature，root activity gradually increased and the free proline content after 20 d rose
and began to drop at the 50th d. MDA content decreased gradually and then increased，was lower at the 20th d and
the 45th d. Banana adventitious bud grew best at 30℃. At the 25th day under this condition，the root activity reached
the highest（ 11.92 mg /g·h），the contents of free praline（ 58.76 μg/g） and MDA（ 7.15 nmol/g） were low，and
vigorous growing plantlets（ height 5.02 cm，leaf numbers 3.64，root numbers 4.63±0.06，root length 4.91
cm） were most suitable for transplanting. At the 45th day under this condition，the root activity reached higher
（2.33 mg /g·h），the contents of free praline（ 94.83 μg/g） and MDA（ 9.83 nmol/g） were low，and vigorous
growing plantlets（ height 6.15 cm，leaf numbers 4.97，root numbers 6.01，root length 6.06 cm） were suitable for
transplanting. The results suggested that 30℃ was most suitable for the growth of banana adventitious bud and the
most suitable time for transplanting was to train for 25 days. |
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