| 周连宁1，2，郝文龙2，吴属连2，李 锟2，王 波1.不同加速溶剂萃取条件对小球藻油脂提取的影响研究[J].广东农业科学,2016,43(7):126-132 |
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不同加速溶剂萃取条件对小球藻油脂提取的影响研究 |
| Effects of different accelerated solvent extraction conditions on lipids extraction from Chlorella sorokiniana |
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| DOI：10.16768/j.issn.1004-874X.2016.07.020 |
| 中文关键词: 小球藻 加速溶剂萃取法 响应面 生物柴油 |
| 英文关键词: Chlorella sorokiniana accelerated solvent extraction response surface methodology biodiesel |
| 基金项目:国家自然科学基金（513080061006239）；深圳市科技计划项目（JCYJ20140703164828240，JCYJ20140829093714177） |
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| 中文摘要: |
| 采用响应曲面法对加速溶剂萃取法提取小球藻油脂过程的影响因素（萃取温度、萃取时间、氯仿- 甲醇体积比）进行研究。在试验数据的基础上，通过方差分析和多项式回归构建拟合度良好的模型，模型显著（P<0.0001），失拟项不显著（P=0.0821），R2=0.9917，R2adj=0.9811，能客观科学地反映小球藻油脂的真实萃取情况。方差分析还表明，萃取时间对油脂提取量的影响达到极显著水平（P<0.0001），萃取温度达到显著水平（P=0.0496），氯仿- 甲醇体积比则不显著（P=0.5792）。椭圆形的等高线表明因素间存在两两相互作用，结合方差分析发现，萃取温度和氯仿- 甲醇体积比的交互作
用达到极显著水平（P=0.0057）。模型预测的小球藻油脂最高提取量为16.06%，对应各因素的水平为：萃取温度132.37℃、萃取时间12.79 min、氯仿- 甲醇体积比2.26∶1。此条件下的实际油脂提取量为16.10%，接近预测值。 |
| 英文摘要: |
| Response surface analysis（ Box-Behnken） was carried out to investigate the effects of three factors on lipids extraction from Chlorella sorokiniana using accelerated solvent extraction technique. Factors presented
here were extraction temperature，extraction time and chloroform-methanol volume-ratio. Based on experimental
data，ANOVA and quadratic regression reswts showed that， model was significant（ P ＜ 0.0001），in which lack of
fit was not significant and R2 was 0.9917，close to 1 and R2ad（j 0.9811）. ANOVA also indicated that the effects of
extraction time and extraction temperature on lipids extraction were significant，while that of chloroform-methanol
volume-ratio was not. Elliptic contour plots showed the presence of interaction between factors. The highest amount of extract of predicted and experimental values were 16.06% and 16.10%，corresponding condition in which extraction
temperature was 132.37℃，extraction time was 12.79 min and chloroform-methanol volume-ratio was 2.26∶1. |
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