| 卢许波，赵 阳，德吉央金，格桑卓玛，德 吉.拉萨扎叶巴沟瑞香狼毒根际土壤细菌的分离与鉴定[J].广东农业科学,2016,43(10):95-99 |
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拉萨扎叶巴沟瑞香狼毒根际土壤细菌的分离与鉴定 |
| Isolation and identification of bacteria from the rhizosphere soil of Stellera chamaejasme L. in Zhayeba Valley，Lhasa |
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| DOI：10.16768/j.issn.1004-874X.2016.10.017 |
| 中文关键词: 拉萨 扎叶巴沟 瑞香狼毒 土壤 26S rDNA D1/D2 区域序列分析 |
| 英文关键词: Lhasa Zhayeba Valley Stellera chamaejasma soil sequence analysis of the D1/D2 domain of 26S rDNA |
| 基金项目:西藏国家级大学生创新实验训练计划项目（201510694006） |
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| 中文摘要: |
| 开展瑞香狼毒（Stellera chamaejasma）根际土壤微生物的研究，能够为遏制草原上瑞香狼毒的
肆虐提供参考资料。釆用稀释倒平板法从瑞香狼毒根际土壤中分离、纯化细菌；采用经典分类和26S rDNA
D1/D2区域序列分析相结合的技术，对纯化的菌株进行鉴定。结果显示，分离自拉萨河谷（扎叶巴沟）的瑞
香狼毒土壤中的细菌55株，归属为鞘氨醇单胞菌（Sphingomonas melonis）、Pseudomonas sp.、蜡状芽孢杆菌
（Bacillus cereus）、日本假单胞菌（Pseudomonas japonica）、芽孢杆菌（Bacillus sp.），其中又以鞘氨醇单胞
菌为优势种；扎叶巴沟瑞香狼毒根际土壤细菌的种群结构间存在一定差异。其中鞘氨醇单胞菌对瑞香狼毒
的抑制作用值得进一步研究。 |
| 英文摘要: |
| Study on the microorganisms from rhizosphere soil of Stellera chamaejasme L. can provide reference
for the suppression of rampant S. chamaejasme L. Bacteria from the rhizosphere soil of Stellera chamaejasme L. were
isolated and purified by dilution plate method，and the purified bacterial strains were identified using classical
classification method combined with sequence analysis of the D1/D2 domain of 26S rDNA；data processing and
analysis were conducted by Microsoft Excel and SPSS 19.0 software. Results demonstrated that 55 bacterial strains
isolated from the soil of S. chamaejasme L. in Lhasa river valley（ Zhayeba valley） belonged to Sphingomonas
melonis，Pseudomonas sp.，Bacillus cereus，Pseudomonas japonica and Bacillus sp.，among which Sphingomonas
melonis was a dominant species. Additionally，there was a certain difference in the population structure of bacteria
from the rhizosphere soil of S. chamaejasme L. in Zhayeba valley. In conclusion，the inhibitory effect of S. melonis on S.
chamaejasme L. is worthy of further study. |
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