| 陈媛媛1，谭海文2，卢燕回3，黎起秦1，林 纬1，袁高庆1.广西烟草立枯病菌和靶斑病菌菌丝融合群初步分析[J].广东农业科学,2016,43(10):106-111 |
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广西烟草立枯病菌和靶斑病菌菌丝融合群初步分析 |
| Anastomosis groups of Rhizoctonia solani causing tobacco sore shin and target spot in Guangxi |
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| DOI：10.16768/j.issn.1004-874X.2016.10.019 |
| 中文关键词: 烟草立枯病 烟草靶斑病 立枯丝核菌 菌丝融合群 |
| 英文关键词: tobacco sore shin tobacco target spot Rhizoctonia solani anastomosis groups |
| 基金项目:中国烟草总公司广西壮族自治区公司科技创新项目（2014-07） |
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| 摘要点击次数: 1915 |
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| 中文摘要: |
| 为了明确广西烟草立枯病菌和靶斑病菌的菌丝融合群，从广西发病烟草植株上分离了2株烟
草立枯病菌菌株和3株靶斑病菌菌株。经形态特征和细胞核染色观察发现，这5株菌株均属于多核立枯
丝核菌。依据供试菌株与标准菌株的菌丝融合反应，发现广西烟草立枯病菌和靶斑病菌均包含AG-2和
AG-4两个菌丝融合群。进一步分析5个菌株的rDNA-ITS序列，初步明确这2种病原菌包括AG-4 HG-I
和AG-2-2 IIIB两个菌丝融合群，与菌丝融合反应分析结果相吻合。首次在国内从烟草靶斑病组织上分离
到AG-2和AG-4融合群。 |
| 英文摘要: |
| In order to investigate the anastomosis groups of the pathogens causing tobacco sore shin and target
spot in Guangxi，two strains were isolated from diseased basal stems and three strains were isolated from diseased
leaves. Based on morphological characteristics and nuclear staining，five strains were identified as Rhizoctonia solani
and they all belonged to multinucleate. Through hyphal anastomosis reactions between tested strains and standard
strains，the pathogens of tobacco sore shin and target spot in Guangxi were all divided into two anastomotic groups:
AG-2 and AG-4. Moreover，ITS sequences analysis of the five strains indicated that the two anastomosis groups were
AG-4 HG-I and AG-2-2 IIIB，and it was in accord with the result of hyphal anastomosis reaction. AG-2 and AG-4
were firstly found in tobacco leaf in China． |
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