| 秦 楚1，李 昱2，张喜斌1，麻冬梅3，许 兴3.苜蓿高频再生组织培养体系的优化[J].广东农业科学,2016,43(11):22-26 |
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苜蓿高频再生组织培养体系的优化 |
| Optimization of high-frequncy regeneration system of Alfalfa |
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| DOI：10.16768/j.issn.1004-874X.2016.11.004 |
| 中文关键词: 苜蓿 激素 愈伤组织 再生体系 |
| 英文关键词: Medicaho sativa L. hormones callus regeneration system |
| 基金项目:宁夏自然科学基金（NZ15002） |
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| 摘要点击次数: 1842 |
| 全文下载次数: 740 |
| 中文摘要: |
| 以3 个品种紫花苜蓿7 日龄无菌苗的下胚轴为外植体，以MS 培养基为基础培养基，研究
不同浓度激素配比对紫花苜蓿离体再生体系中愈伤组织的形成、不定芽诱导以及生根的影响。结果表
明：金皇后、甘农1 号、阿尔冈金愈伤组织诱导的最佳培养基分别为MS+2，4-D 2.5 mg/L+6-BA 0.5
mg/L、MS+2，4-D 2.0 mg/L+NAA 1.0 mg/L、MS+2，4-D 3.0 mg/L+KT 1.0 mg/L；不定芽诱导的最佳培养基分
别为MS+6-BA 2.0 mg/L+CH 0.5 g/L、MS+NAA 0.3 mg/L+KT 0.5 mg/L、MS+KT 1.2 mg/L；最佳生根培养基为
1/2MS+IBA 1.0 mg/L。 |
| 英文摘要: |
| Hypocotyls of 3 varieties of Alfalfa in 7 days old seedlings were used as explants，Ms medium
was used as basic medium，the effects of different concentrations of auxins on Alfalfa callus regeneration
system，adventitious bud induction and rooting were studied. The results indicated that， for Golden
Empress，Gan-nong No.3 and Algonquin，the best mediums for callus induction were MS+2，4-D 2.5
mg/L+6-BA 0.5 mg/L，MS+2，4-D 2.0 mg/L+NAA 1.0 mg/L，MS+2，4-D 3.0 mg/L+KT 1.0 mg/L；the best
differentiation mediums were MS+6-BA 2.0 mg/L+CH 0.5 g/L，MS+NAA 0.3 mg/L+KT 0.5 mg/L，MS+KT 1.2 mg/L；
the best rooting medium for plant development were 1/2MS+IBA 1.0 mg/L. |
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