| 黎 宁1，2，王金生1，王业耀1，3，王学礼4，秦旭芝2.镍对蕹菜生长和营养成分的影响[J].广东农业科学,2017,44(3):52-60 |
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镍对蕹菜生长和营养成分的影响 |
| Effects of nickel on growth and nutritional ingredientsof water spinach（ Ipomoea aquatica Forsk.） |
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| DOI：10.16768/j.issn.1004-874X.2017.03.008 |
| 中文关键词: 水培 蕹菜 施镍处理 脲酶 氨基酸 可溶性糖 |
| 英文关键词: hydroponic water spinach nickel treatment urease amino acid soluble carbohydrates |
| 基金项目:广西科学研究与技术开发计划项目（桂科合14125008-2-30）；广西农业重点科技计划项目
（201519）；环境保护部公益项目（ 201309032）；广西土壤环境污染与生态修复人才小高地项目；广西西江流域生态
环境与一体化发展协同创新中心项目 |
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| 中文摘要: |
| 通过水培方式种植蕹菜，研究以尿素态氮作唯一氮源条件下，不同浓度镍对蕹菜生长、脲酶活
力和某些营养成分的影响。结果表明：适量镍对蕹菜生长、脲酶活力和氨基酸合成有促进作用，但过量的镍
产生毒害作用。施镍20 μg/L 处理的蕹菜鲜重最高、比对照高12.1%，但镍浓度高于320 μg/L 时蕹菜鲜重
下降，施镍1 280 μg/L 处理的蕹菜表现出受害症状；适量的镍（20～80 μg/L）可显著提高蕹菜叶片的脲酶
活力，最高幅度达13.6%，脲酶含量也显著提高，最大增幅19.0%，但过量镍（大于640 μg/L）会抑制脲酶
活力。研究足量镍（60 μg/L）影响蕹菜脲酶的作用机理，结果显示，镍可能先是提高脲酶比活力，然后参
与脲酶合成而提高脲酶含量，从而更进一步提高脲酶活力。叶片中总氨基酸含量在镍0～40 μg/L 范围内随
镍浓度提高而升高，最高升幅44.7%，镍大于10 μg/L 时效果极显著，之后保持稳定水平；叶片中可溶性糖
含量在过高浓度镍处理（大于640 μg/L）时显著升高。 |
| 英文摘要: |
| The paper investigated the effects of various concentrations of nickel on growth，urease activity and
some nutritional ingredients of hydroponic water spinach by supplying urea nitrogen as the only nitrogen source.
The results indicated that appropriate quantity of nickel could enhance the growth，urease activity and amino acids
synthesis of water spinach，while excessive nickel was toxic to water spinach. The treatment of 20 μg/L nickel
resulted in the highest fresh weight of water spinach，12.1% higher than that in control，while significant reduction
of water spinach fresh weight was detected with nickel supply higher than 320 μg/L，and significant plant damage
symptoms were observed with 1 280 μg/L nickel treatment. This results also showed that appropriate concentrations
of nickel（ 20-80 μg/L） could significantly increase the urease activity up to 13.6%，while the urease content also
raised up to 19.0%，but excessive nickel supply（ more than 640 μg/L） inhibited the urease activity. Physiological
analysis demonstrated that adequate nickel concentration（ 60 μg/L） firstly enhanced the specific activity of urease，
then participated in the urease synthesis to improve the urease yield，and ultimately improved the overall urease activity. This results also identified that the total amino acids content increased simultaneously as the concentrations
of nickel raised within the range of 0-40 μg/L with a highest increase of 44.7%，this effect became significant and
stable with nickel over 10 μg/L；the content of soluble carbohydrates in leaves significantly increased with treatments
of over-concentrated nickel（ more than 640 μg/L）. |
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