文章摘要
董章勇,罗 梅,向梅梅.茄科尖孢镰刀菌3 个专化型细胞壁降解酶的比较[J].广东农业科学,2017,44(5):112-117
查看全文    HTML 茄科尖孢镰刀菌3 个专化型细胞壁降解酶的比较
Comparison of cell wall degrading enzymes from three different Fusarium oxysporum formae speciales ofSolanaceae
  
DOI:10.16768/j.issn.1004-874X.2017.05.018
中文关键词: 茄科枯萎病  尖孢镰刀菌  专化型  细胞壁降解酶
英文关键词: Fusarium wilt disease of Solanaceae  Fusarium oxysporum  formae speciale  cell wall degrading enzymes
基金项目:国家自然科学基金(31301627);广东省自然科学基金(S2012040006912)
作者单位
董章勇,罗 梅,向梅梅 仲恺农业工程学院植物保护系广东 广州 510225 
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中文摘要:
      对茄科作物致病尖孢镰刀菌番茄专化型(Fusarium oxysporum f. sp. lycopersici,FOL)、茄子专化 型(F. oxysproum f. sp. melongenae,FOM)和辣椒专化型(F. oxysporum f. sp. capsicum,FOC)细胞壁降解酶 多聚半乳糖醛酸酶(PG)、果胶甲基半乳糖醛酸酶(PMG)、多聚半乳糖醛酸反式消除酶(PGTE)、果胶甲 基反式消除酶(PMTE)及纤维素酶(Cx)进行比较。对3 个专化型分别在寄主植物体内细胞壁降解酶活 性测定表明:发病茄子和番茄体内酶活性从高到低依次为PG、PMG、PGTE、Cx 和PMTE,发病辣椒体内酶 活性从高到低依次为PG、PGTE、PMG、PMTE 和Cx。体外诱导试验发现,FOC 所产生的PG 活性比FOM 和 FOL 高,而FOM 所产生的PGTE 比FOC 和FOL 高,3 个专化型的PMG、PMTE 和Cx 活性没有显著差异。 通过PG 同工酶薄层等电聚焦电泳分析,FOL、FOM 和FOC 分别产生5、5、3 个PG 同工酶,各有1 条特异 的PG 同工酶条带。推测此3 个专化型PG 同工酶的差异可能和病原菌的致病作用和寄主专化性差异有关。
英文摘要:
      We compared the cell wall degrading enzymes,including polygalacturonase( PG),pectin methylgalacturonase( PMG),polygalacturonic acid transeliminase( PGTE),pectin methyl transeliminase( PMTE) and cellulose( Cx),from three different Fusarium oxysporum formae speciales of Solanaceae as F. oxysporum f. sp. lycopersici( FOL),F. oxysproum f. sp. melongenae( FOM),and F. oxysporum f. sp. capsicum( FOC). We found that the higher to lower enzyme activity order were PG,PMG,PGTE,Cx and PMTE in inoculated eggplant or tomato in planta,while an order of PG,PGTE,PMG,PMTE and Cx in inoculated capsicum. We also found that PG activity of FOC was higher than that of FOM or FOL in vitro. There was no significant difference in the activity of PMG,PMTE and Cx in these three fungal in vitro. Analysis of PG isoenzymes by IEF-PAGE showed that FOL,FOM and FOC could secrete a total of 13 PGs,with 5,5,and 3 PG isoenzymes,respectively. Each fungus had a specific PG band. These results will provide useful information for managing Fusarium wilt and insights into pathogenic factors for phytopathology.
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