| 贾爱娟1，刘 姚2，张 挺3，沈 兴2.基于3 种载体蛋白的莱克多巴胺抗原的合成与检测[J].广东农业科学,2017,44(7):125-131 |
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基于3 种载体蛋白的莱克多巴胺抗原的合成与检测 |
| Synthesis and testing the ractopamine antigens conjugating with three different carrier proteins |
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| DOI：10.16768/j.issn.1004-874X.2017.07.020 |
| 中文关键词: 莱克多巴胺 载体蛋白 半抗原 人工抗原 免疫原性 |
| 英文关键词: ractopamine carrier protein hapten artificial antigen immunogenicity |
| 基金项目:国家自然科学基金（31601555） |
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| 摘要点击次数: 1699 |
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| 中文摘要: |
| 为制备高效莱克多巴胺（Ractopamine，RAC）抗体，将人工合成的RAC- 琥珀酸酯半抗原与3
种载体蛋白（BSA、OVA 和KLH）偶联合成人工抗原，采用紫外光谱扫描、测定偶联比和ESI-MS 进行鉴
定；通过免疫小鼠获得抗血清，采用ELISA 比较其效价，对3 种人工抗原的免疫原性进行评定，结果显示3
种抗血清效价为4 000～32 000，表明合成的3 种人工抗原都具有免疫原性，其中以KLH 为载体蛋白的抗原
的免疫效果较好，可用于制备高效RAC 抗体。 |
| 英文摘要: |
| In order to prepare anti-RAC antibody with high affinity，the synthesized hapten RAC-succinic acid
ester was conjugated with three different carrier proteins（ BSA，OVA，KLH）. After identified by UV spectroscopy
scanning，ESI-MS and conjugation ratio，the successfully synthesized artificial antigens were used to immunize mouse.
The titers of the obtained antiserum was detected by ELISA. The titers of the three artificial antigens were 4 000-32 000.
The results showed that the three artificial antigens had satisfactory immunogenicity，and the immunogenicity of KLH
was better than that of BSA and OVA. Therefore，the antigen can be used to prepare high affinity antibody. |
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