| 曾武清1，陈柳婵2，曾瑞珍2，易懋升1，宿庆连1，郭和蓉2，黎扬辉1，谢 利2.丽影和红韵蝴蝶兰快速繁殖试验[J].广东农业科学,2017,44(8):55-60 |
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丽影和红韵蝴蝶兰快速繁殖试验 |
| Micropropagation of Phalaenopsis‘ Liying’ and‘ Hongyun’ |
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| DOI：10.16768/j.issn.1004-874X.2017.08.009 |
| 中文关键词: 蝴蝶兰 新品种 组织培养 快速繁殖 丛生芽 |
| 英文关键词: Phalaenopsis new cultivar tissue cutire micropropagation clump shoot |
| 基金项目:广州市科技计划项目（2014Y2-00125）；广州市农业财政专项资金项目（1510170，穗财农[2016]26 号） |
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| 中文摘要: |
| 以花梗腋芽为材料，研究了蝴蝶兰新品种丽影和红韵的快速繁殖技术。结果表明，消毒时间对
营养芽诱导率有显著影响，0.1%HgCl2 消毒8 min 的红韵蝴蝶兰营养芽诱导率最高，激素对营养芽诱导率影
响不显著。6-BA 浓度、有机附加物和芽数对丛生芽增殖影响显著，当6-BA 浓度为8.0 mg/L 时，丽影和红
韵芽增殖系数最高，分别为2.31 和2.22；添加椰汁更有利于减轻褐变，提高增殖系数；单芽接入的增殖系
数最高。激素对生根率无显著影响，但对平均根数和苗高有显著影响，在含6-BA 0.1 mg/L ＋ NAA 1.0 mg/L
的培养基上试管苗株高最高，平均根数最多。以松树皮为基质，丽影和红韵蝴蝶兰试管苗移栽成活率分别
为91.58% 和87.27%。 |
| 英文摘要: |
| Micropropagation of new cultivars of Phalaenopsis‘ Liying’ and Phalaenopsis‘ Hongyun’ was
investigated by using of peduncle as explant. The results indicated that disinfection time had significant effect on the
induction rate of vegetative shoot，after disinfected with 0.1% HgCl2 for 8 minutes，the induction rate of vegetative
bud was the highest，while exogenous hormones had no significant effect on the induction rate of Phalaenopsis
‘Hongyun’. The concentration of 6-BA，organic additives and the number of bud in an inoculums had significant
influence on bud proliferation. At the concentration of 6-BA being 8 mg/L，the proliferation coefficients of
Phalaenopsis‘ Liying’ and Phalaenopsis‘ Hongyun’ were the highest，accounting for 2.31 and 2.22，respectively.
Supplying coconut milk in proliferation medium was more beneficial for alleviating the browning and enhancing the
proliferation coefficient compared with adding potato or banana. Single bud as an inoculum favored bud proliferation
and enhanced the multiplication coefficient. Exogenous hormone had little effect on the root induction rate，but had
significant effect on the mean number of root and height of plantlet. Seedling height of Phalaenopsis‘ Hongyun’ and
Phalaenopsis‘ Liying’ was the highest and the mean number of root was the most on modified 1/2MS medium with
6-BA 0.1 mg/L + NAA 1 mg/L. By using pine bark as culture substrate，the survival rates of Phalaenopsis‘ Liying’
and Phalaenopsis‘ Hongyun’ were 91.58% and 87.27%，respectively. |
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